Table 1 Primer sets for three genera of free-living amoebae were used in the present study
Free-living amoeba | Primer’s name | Target gene | Primers 5´ → 3´ | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
Acanthamoeba spp. | JDP | SSU rRNA | GGCCCAGATCGTTTACCGTGAA TCTCACAAGCTGCTAGGGAGTCA | ~500 | [36] |
Vahlkampfiids | ITS | Internal Transcribed Spacer (ITS) | GAACCTGCGTAGGGATCATTT TTTCTTTTCCTCCCCTTATTA | ~400–700 | [37] |
Vermamoeba vermiformis (formerly named Hartmannella vermiformis) | Hv | 18 S ribosomal RNA | TTACGAGGTCAGGACACTGT GACCATCCGGAGTTCTCG | ~500 | [38] |