Table 1 Primers used for gene expression in tissues of Clinostomum-infected fish
Genes | Primers sequences Forward (5'–3') | Primers sequences Reverse (5'–3') | Reference |
|---|---|---|---|
IL-1β | AACACTGACAGAACAACTGCGAACA | TCGCAGTTGTTCTGTCAGTGTTGTT | [20] |
MHC-Ia | TTCTCACCAACAATGACGGG | AGGGATGATCAGGGAGAAGG | |
MHC-II | AGTGTGGGGAAGTTTGTTGGAT | ATGGTGACTGGAGAGAGGCG | |
β-Actin | GAGCGTGAGATTGTGCGTGAC | TCCATACCGAGGAATGAGGGC |