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Table 2 Calves sera reactivity in the IgM and IgG1 class with evaluated proteins in ELISA

From: Subcutaneous application of hyperimmune serum against Histophilus somni recombinant proteins affects serum antibody reactivity in beef calves

  

Herd

CH

LM

MIX

Protein

Ig class

Sampling

C

n = 18

E

n = 18

C

n = 17

E

n = 17

C

n = 9

E

n = 8

rHsp60

IgM

1

1.25

(0.97–1.60)

1.38

(1.11–1.67)

1.04

(0.67–1.31)

0.77

(0.49–1.23)

0.77

(0.53–0.90)

0.87

(0.47–1.07)

2

0.44

(0.34–0.51)

0.47

(0.41–0.53)

0.27

(0.23–0.31)

0.26

(0.21–0.30)

0.24

(0.08–0.29)

0.29

(0.09–0.34)

3

1.27

(0.97–1.87)

1.30

(1.10–1.52)

1.61

(1.42–2.01)

1.81

(1.52–2.12)

1.94

(1.19–2.61)

2.55

(2.00–3.00)

rOMP40

1

0.70

(0.57–1.03)

0.90

(0.66–1.36)

0.58

(0.40–0.81)

0.49

(0.41–0.55)

0.42

(0.37–0.47)

0.53

(0.33–0.59)

2

0.34

(0.30–0.42)

0.38

(0.31–0.53)

0.28

(0.23–0.31)

0.30

(0.24–0.33)

0.25

(0.21–0.26)

0.21

(0.18–0.31)

3

0.87

(0.70–1.05)

0.87

(0.71–1.17)

1.02

(0.91–1.13)

1.06

(0.93–1.25)

1.64

(0.94–2.24)

1.47

(0.91–1.97)

rHsp60

IgG1

1

1.24

(1.06–1.58)

1.37

(0.99–1.78)

0.93

(0.62–1.52)

0.80

(0.59–1.14)

0.83

(0.59–0.92)

0.66

(0.43–1.11)

2

0.87

(0.62–1.18)

1.04

(0.93–1.42)

0.69

(0.52–0.88)

0.82

(0.61–1.06)

0.46

(0.33–1.08)

0.67

(0.43–0.99)

3

0.84

(0.57–1.29)

0.71

(0.43–1.35)

1.42

(1.00-1.70)

1.04

(0.81–1.48)

0.69

(0.56–0.75)

0.48

(0.34–0.64)

  1. Legend: Median (Q1-Q3) optical density. rHsp60, rOMP40- H. somni recombinant proteins. Herds (dominant cows breed) CH- Charolaise, LM-limousine, MIX crossbreed beef and dairy breed. C- control, E- experimental