Table 1 Median (mean ± SD) cows’ serum reactivity with recombinant proteins in ELISA during hyperimmunization

rHsp60

IgG1

0.15

(0.15 ± 0.0)

0.26*

(0.29 ± 0.1)

1.02*

(1.00 ± 0.3)

1.25*

(1.31 ± 0.3)

1.24*

(1.23 ± 0.1)

1.24*

(1.29 ± 0.3)

0.93*

(1.13 ± 0.3)

IgG2

0.14

(0.14 ± 0.0)

0.33*

(0.30 ± 0.1)

1.05*

(1.09 ± 0.5)

1.50*

(1.46 ± 0.5)

1.68*

(1.61 ± 0.5)

1.62*

(1.65 ± 0.2)

1.96*

(1.71 ± 0.6)

IgM

0.82

(0.79 ± 0.2)

0.82

(0.81 ± 0.2)

0.82

(0.85 ± 0.1)

0.81

(0.81 ± 0.1)

0.85

(0.87 ± 0.1)

0.87

(0.89 ± 0.2)

0.68

(0.69 ± 0.1)

rOMP40

IgG1

0.15

(0.15 ± 0.0)

0.32*

(0.44 ± 0.4)

0.99*

(1.03 ± 0.2)

0.90*

(1.02 ± 0.4)

1.20*

(1.09 ± 0.3)

1.15*

(1.14 ± 0.3)

1.16*

(1.13 ± 0.2)

IgG2

0.16

(0.19 ± 0.1)

0.29*

(0.77 ± 1.0)

1.27*

(1.28 ± 0.7)

1.29*

(1.32 ± 0.7)

1.31*

(1.31 ± 0.5)

1.36*

(1.35 ± 0.7)

1.47*

(1.37 ± 0.4)

IgM

0.53

(0.52 ± 0.2)

0.65

(0.72 ± 0.2)

0.79

(0.91 ± 0.4)

0.77

(0.92 ± 0.4)

1.11

(0.98 ± 0.3)

0.95

(0.94 ± 0.4)

0.76

(0.92 ± 0.3)