Skip to main content
Fig. 8 | BMC Veterinary Research

Fig. 8

From: Genome-wide transcriptional profiling and functional analysis of long noncoding RNAs and mRNAs in chicken macrophages associated with the infection of avian pathogenic E. coli

Fig. 8

Construction and activity verification of TCONS_00007391 RNA interference/overexpression vector. A Double enzyme digestion of the TCONS_00007391 interference recombination plasmid. M, Marker; 1, empty vector; 2–3, shRNA1; 4–5, shRNA2; 6–7, shRNA3. B Sanger sequencing of the TCONS_00007391 interference recombination plasmid. C Relative expression of TCONS_00007391 in macrophages transfected with shRNA vectors for 48 h as measured by RT-qPCR. D Bacterial liquid PCR amplification of TCONS_00007391. M, Marker; 1–6, TCONS_00007391. E Relative expression of TCONS_00007391 in macrophages transfected with overexpression vector for 48 h as measured by RT-qPCR

Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us