Fig. 10

TCONS_00007391 was able to regulate avian pathogenic E. coli (APEC) induced cell viability via targeting CD86. A The morphology of chicken macrophages in the groups of Control, APEC, ovexpression of TCONS_00007391 + APEC, and knockdown of TCONS_00007391 + APEC. B The cell viability of chicken macrophages in the groups of Control, APEC, ovexpression of TCONS_00007391 + APEC, and knockdown of TCONS_00007391 + APEC. Data are shown as mean ± SD; n = 4 independent experiments; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. C Luciferase reporter assay was used to determine the relationship between knockdown of TCONS_00007391 and CD86. Firefly luciferase activities were normalized to Renilla luciferase activities. Data are shown as mean ± SD; n = 4 independent experiments; ** p < 0.01, **** p < 0.0001. D RT-qPCR analysis of CD86 mRNA expression in macrophages transfected with the TCONS_00007391 knockdown plasmid. Data are shown as mean ± SD; n = 4 independent experiments; ** p < 0.01, **** p < 0.0001. E Luciferase reporter assay was used to determine the relationship between overexpression of TCONS_00007391 and CD86. Firefly luciferase activities were normalized to Renilla luciferase activities. Data are shown as mean ± SD; n = 4 independent experiments; ** p < 0.01, **** p < 0.0001. F RT-qPCR analysis of CD86 mRNA expression in macrophages transfected with the TCONS_00007391 overexpression plasmid. Data are shown as mean ± SD; n = 4 independent experiments; **** p < 0.0001