Comparison of Sysmex XN-V body fluid mode and deep-learning-based quantification with manual techniques for total nucleated cell count and differential count for equine bronchoalveolar lavage samples

Table 7 Comparison of precision for two-part differential count between automated Sysmex BF differential after regating and manual 200-cell differentiation with light microscopy

Method	Sample cellularity	Cell type	Cell type %	n	SD (%)	CV (%)
Sysmex BF	Low^a	MN%	81.7	10	1.61	2.0
	Low^a	PMN%	18.4	10	1.35	7.3
	Moderate^a	MN%	92.2	10	0.71	0.8
	Moderate^a	PMN%	7.8	10	0.71	9.0
	High^a	MN%	34.8	10	0.60	1.7
	High^a	PMN%	65.2	10	0.60	0.9
Manual	Low^a	MN%	81.3	10	4.03	5.0
	Low^a	PMN%	16.7	10	3.86	23.1
	Moderate^a	MN%	94.3	10	1.64	1.7
	Moderate^a	PMN%	5.6	10	1.71	30.6
	High^a	MN%	26.1	10	6.97	26.7
	High^a	PMN%	73.9	10	6.97	9.4

BF Body fluid, CV Coefficient of variation, MN Mononuclear, n Number of replicates, PMN Polymorphonuclear, SD Standard deviation, TNCC Total nucleated cell count
^amean manual TNCC, where low = 42.5 × 10⁶ cells/µL, moderate = 252.5 × 10⁶ cells/µL, high = 997.5 × 10⁶ cells/µL

ISSN: 1746-6148