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Table 3 Summary of ANV detection and quantification results in enteric samples from chickens with RSS

From: Development of a fast and sensitive RT-qPCR assay based on SYBR® green for diagnostic and quantification of Avian Nephritis Virus (ANV) in chickens affected with enteric disease

RT-qPCR Results for ANV detection and quantification

Birds

Age

Total samples

Positive samples

Average of GC/µL RNA

Maximum GC/µL RNA

Broilers

Days

1–7

200

16/18

34,937

482,513

 > 7–21

11/17

52,924

561,785

 > 21–41

21/29

499

5329

 > 41

74/77

3298

95,241

Breeders hens

Weeks

1

16/21

371,997

5,538,297

 > 1–30

3/12

77

138

 > 30–44

10/11

16,193

128,545

 > 44

13/15

666,310

3,712,673