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Table 2 Repeatability assays using g-Block dilutions from 108 to 104 copies of genetic material

From: Development of a fast and sensitive RT-qPCR assay based on SYBR® green for diagnostic and quantification of Avian Nephritis Virus (ANV) in chickens affected with enteric disease

Copy Number

Inter-Assay

Intra-Assay

Cq Mean

Cq St Dev

Cq Mean

Cq St Dev

108

14,424

0,743

13.965

0,430

107

19,661

0,765

16,932

0,303

106

21,768

0,976

21,20

0,094

105

25,024

0,767

24,21

0,107

104

29,781

0,387

27,18

0,085

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BMC Veterinary Research

ISSN: 1746-6148

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