Development of a fast and sensitive RT-qPCR assay based on SYBR® green for diagnostic and quantification of Avian Nephritis Virus (ANV) in chickens affected with enteric disease

Table 1 Primers used in this study

Primer	Gene	Assay	Sequences	Product	Reference
ANV-F	ORF1b	qPCR	5’-CCTTTCYAACCAGATAARGCGTG -3’	86 bp	This study
ANV-R	ORF1b	qPCR	5’-TTCTGTAGAAGTCGGGCCCG -3’	86 bp	This study

ISSN: 1746-6148