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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Development of a fast and sensitive RT-qPCR assay based on SYBR® green for diagnostic and quantification of Avian Nephritis Virus (ANV) in chickens affected with enteric disease

Fig. 3

Phylogenetic relationships between the sequences of ANV obtained here and other sequences of ANV from Australia, Brazil, China, Israel, Japan, Switzerland, the United Kingdom, and the United States based on a part of ORF 1b gene NT sequences. Sequences were aligned using the CLUSTAL W method in ClustalX2 2.1. The phylogenetic tree was inferred using MEGA7 software on the alignments of the partial ORF 1b sequences of ANV using a Phylogeny reconstruction with a Neighbor-joining Statistical Method joined with a p-distance model and 1000 bootstraps of replication. The tree showed the phylogenetic relationships of the Ecuadorian ANV sequences with others present in GenBank. The numbers along the branches indicate the bootstrap value for every 1000 replicates. The scale bar represents the number of substitutions per site. The Chicken Parvovirus sequence (MK358350.1) was used as an outgroup. ■ = Sequences obtained in this study and written in blue

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BMC Veterinary Research

ISSN: 1746-6148

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