Table 2 Secondary antibodies employed in this current investigation
Conjugate | Species | Antigen | Dilution | Supplier |
|---|---|---|---|---|
1. Alexa fluor 488 | Donkey | Rabbit IgG | 1:500 | Thermo Fisher Scientific Waltham, MA, USA |
2. Alexa fluor 488 | Donkey | Rat IgG | 1:500 | Thermo Fisher Scientific Waltham, MA, USA |
3. Alexa fluor 488 | Donkey | Goat IgG | 1:500 | Thermo Fisher Scientific Waltham, MA, USA |
4. Cyanine 3 | Donkey | Rabbit IgG | 1:400 | Merck Millipore Massachusetts, USA |
5. Cyanine 3 | Donkey | Mouse IgG | 1:400 | Merck Millipore Massachusetts, USA |