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Fig. 9 | BMC Veterinary Research

Fig. 9

From: The tongue of the red-eared slider (Trachemys scripta elegans): morphological characterization through gross, light, scanning electron, and immunofluorescence microscopic examination

Fig. 9

Double immunofluorescence staining for SOX9 (Green) and PDGFRα (Red) with DAPI (Blue) in the tongue root of the red-eared slider. The tongue of the red-eared slider contains longitudinal hyaline cartilage in the body and circular hyaline cartilage in the root. Panels A-C depict the longitudinal lingual hyaline cartilage (LHC), chondrocytes (Ch), and perichondrium (F). These chondrocytes express SOX9 and PDGFRα. Panels D-F provide a higher magnification of the A-C view, highlighting SOX9 and PDGFRα-positive chondrocytes within the longitudinal lingual hyaline cartilage. Furthermore, Panels G-I depicted the circular lingual hyaline cartilage (CHC), chondrocytes (Ch), and perichondrium (F). Like the longitudinal cartilage, chondrocytes within the circular hyaline cartilage also express SOX9 and PDGFRα. White dotted lines have been added to panels A, D, and G to delineate the shape of the longitudinal and circular hyaline cartilage. Scale bars: A-C = 100 μm, D-F = 50 μm, G-I = 100 μm

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