Fig. 4
FPN significantly reduced the mRNA expression of GAD65 and GAT1 and altered the GABA receptor subunit expression. The total RNA of splenocytes (6 ⨉ 106 cells) harvested from different treatment groups was extracted to detect the mRNA expression of GAD65, GAD67, GAT1, GABAR α5, GABAR β2, GABAR β3, and GABAR δ by qPCR. The expression level of HPRT was used as the control for semi-quantification. Results were expressed as the mean ± SEM pooled from four independent experiments with technological duplication in each group (n = 20). *p < 0.05 was significant compared to the VH group