Table 2 Primers used in qRT-PCR
Gene | GenBank accession | Primer sequence (5’-3’) | Tm (°C) | Product length |
|---|---|---|---|---|
SDHC | XM_004002697.4 | F-TCCGACACTTGATGTGGGACCTAG R-ACAACACAGTAAGAACCAGGACAGC | 60.0 | 90 bp |
NDUFS1 | XM_004004855.4 | F-TTTGGGAACTACAGGCAGAGGAAAC R-AAGGCATAGGGCTTAGAGGTCAGG | 60.0 | 107 bp |
MDH2 | XM_004021260.5 | F-GCGATGAACGGAAAGGAAGGAGTC R-AGCAGCAACGGTGTGGAGAAATAC | 60.0 | 89 bp |
CXCL10 | NM_001009191.1 | F-GCATACCTCTCTCTAGGAACACACG R-GGGCAGGATTGACTTGCAGGAATC | 60.0 | 112 bp |
TNF | XM_012100437.4 | F-CAACGGCGTGGAGCTGAAAGAC R-TGAAGAGGACCTGCGAGTAGATGAG | 60.0 | 80 bp |
TYK2 | XM_042249928.1 | F-TGGTGAGATGGTAGCCGTGAAGG R-ACTTGACGATGTGCTTGTGGTAGAG | 60.0 | 116 bp |
ACADL | XM_027965150.2 | F-TTGAAGATGTACGGTTGCCAGCAG R-TGGAAGCTCTTGCATGAGGTAATGG | 60.0 | 80 bp |
HSD17B4 | XM_004008685.4 | F-GAGGTTGGAGCAGGATGGATTGG R-CTCTGAGGCTTGGTGGCATTATCG | 60.0 | 149 bp |
HIBCH | XM_004004498.5 | F-AAAGGCTTTCTGTGCTGGAGGTG R-CTGGCAAGAATCAATGGCGTTGTTC | 60.0 | 124 bp |
GAPDH | NM_001190390.1 | F- CGGCACAGTCAAGGCAGAGAAC R- CACGTACTCAGCACCAGCATCAC | 60.0 | 115 bp |