In vitro and in vivo antitrypanosomal activity of the fresh leaves of Ranunculus Multifidus Forsk and its major compound anemonin against Trypanosoma congolense field isolate

Table 1 In vitro antitrypanosomal effect of the leaf extracts of Ranunculus multifidus and anemonin on Trypanosoma congolense motility and in vivo infectivity test

Dose (mg/ml)	Effect	1% DMSO	DA	RM-M	RM-H	Anemonin
4.0	Change in motility (in min)	NE	10^a	30^b	20^a	5^a
	No of infectious mice / Total no of mice	5/5	0/5	2/5	0/5	0/5
	Infection interval (in days)	12 ± 0.00	Ni	18 ± 0.34	Ni	Ni
2.0	Change in motility (in min)	NE	15^b	45^b	25^b	15^b
	No of infectious mice / Total no of mice	5/5	1/5	3/5	2/5	1/5
	Infection interval (in days)	12 ± 0.00	22	16 ± 0.48	18 ± 0.50	22
0.4	Change in motility (in min)	NE	30^b	50^b	45^b	20^b
	No of infectious mice / Total no of mice	5/5	3/5	5/5	4/5	2/5
	Infection interval (in days)	12 ± 0.00	16 ± 0.56	12 ± 0.00	14 ± 0.37	18 ± 0.00
0.1	Change in motility (in min)	NE	45^b	NE	NE	35^b
	No of infectious mice / Total no of mice	5/5	4/5	5/5	5/5	3/5
	Infection interval (in days)	12 ± 0.00	14 ± 0.48	12 ± 0.00	12 ± 0.00	16 ± 0.37

Data are expressed as mean ± SEM; n = 5; Ni: no infection; DA: diminazene aceturate; 1% dimethyl sulfoxide (DMSO): vehicle; RM-M: 80% methanol extract of Ranunculus multifidus, RM-H: hydro-distilled extract of Ranunculus multifidus; ^amotility ceased; ^bmotility drastically reduced; NE No effect on motility. The experiments were performed in triplicate with each test concentration done in duplicate. The numbers under change in motility means the time (in minute) in which the change of motility is observed

ISSN: 1746-6148