Table 1 Primer sequences for plasmids construction and identification of recombinant virus
From: An improved system to generate recombinant canine distemper virus
Name | Sequence (5′ → 3′) | Orientation | Position |
|---|---|---|---|
F1 | GGAAAGGAATTCCTATAGTCACCAGACAAAGTTGGCTATGGATAG | + | 1–25 |
R1 | CAGACTCAGCCTCATTTGAGGTCCT | - | 2686–2710 |
F2 | CTCAAATGAGGCTGAGTCTGACAGT | + | 2691–2715 |
R2 | AACCAGGTGCACTGAGAGCCTGAGTTG | - | 5264–5290 |
F3 | GGCTCTCAGTGCACCTGGTTAGTCCTG | + | 5371 − 5297 |
R3 | ATAGTACATACCTTGGCTTTGGAATTC | - | 7906–7932 |
F4 | AAAGCCAAGGTATGTACTATAGCAGTGG | + | 7913–7940 |
R4 | GCACTTACGGTTTCATAGATCTCTATA | - | 10,975–11,002 |
F5 | ATCTATGAAACCGTAAGTGCATTTATA | + | 10,983–11,009 |
R5 | CTTTCCAGAAGGTCGGTGATAATGAAT | - | 13,524–13,550 |
F6 | ATCACCGACCTTCTGGAAAGTACCAAA | + | 13,531–13,557 |
R6 | TGGAGATGCCATGCCGACCCACCAGACAAAGCTGGGTATGATAAC | - | 15,666–15,690 |
NF | TAAGCAGAGCTCGGGTACCAGATCTAGGGTCAATGATCCTACCTTAGA | + | 63–78 |
NR | TCTGGATCCCCGCGGCCGCAGATCTGTTTGTTGGACCCGGGTCCTAA | - | 1740–1760 |
PF | TAAGCAGAGCTCGGCTAGCCTCGAGCTTAGGACCCGGGTCCAACAAAC | + | 1745–1761 |
PR | TGCTGGAATTCGGCTTGGGCTCGAGGAGAGGACTTAGGCTCTTGTGT | - | 3400–3421 |
LF: | ATCATTTTGGCAAAGAATTCCTCGAGGGTACCCCCGGGGCGGCCGCAAATGGACTCTGTGTCAGTGAACCAGA | + | 9030–9054 |
LR: | AGGGAAAAAGATCTGCTAGCTAATTAAGAGCTCTTTTTTTTCGTATAACCAAGTTTGATAGC | - | 15,621–15,645 |
PMF | GACGATCATGCGATTGTTTA | + | 8666–8685 |
PMR | TGTCTAATTCGTGCATACTC | - | 9117–9136 |