Fig. 7
Primary culture of murine neurons infected with MHV-JHM after 168 h p.i.. Representative confocal images of specific filamentous actin structures – tunnelling nanotubes (TNTs) (a,a’,a’’,b,b’,c; white arrows); depolymerised (b’ yellow arrow) and highly polymerised rings structures (b’’ yellow arrows) spider-web-like actin structures (c’,c’’ yellow arrows). Green boxes indicate magnified area. Indirect and direct immunofluorescence staining; merge images: actin filaments - red; cell nuclei - blue; viral antigen - green. Microscope magnification 60x, scale 30 μm and 20 μm