Fig. 6

Primary culture of murine neurons infected with MHV-JHM virus. Representative confocal images of neurons obtained at 2 h p.i. (A) and 168 h p.i (B). White asterisk represents the occurrence of a F-actin ring structures – juxtanuclear ring (A) and submembranous ring (B); green boxes show magnified areas; green arrows indicate viral antigen; double yellow arrows show juxtanuclear ring and single yellow arrow points submembrane ring. Indirect and direct immunofluorescence staining; merge images: actin filaments - red; cell nuclei - blue; viral antigen - green. Microscope magnification 60x, scale 20 μm and 10 μm