Fig. 5
Primary culture of murine neurons infected with MHV-JHM virus. Representative confocal images of neurons obtained at 2 (A,a’,a’’), 24 (B,b’,b’’), 48 (C,c’,c’’), 72 (D,d’,d’’), and 168 h p.i (E,e’,e’’). Green arrowheads point to the presence of viral antigens in actin structures. Yellow arrowheads show areas of changes in f-actin filaments resulting from MHV-JHM infection. White arrowheads point to tunnelling nanotube (TNTs) structures. White asterisk show lamellipodia. Yellow boxes indicate the overlapping presence of actin filaments fluorescence with viral antigen and magnified area. Indirect and direct immunofluorescence staining; merge images: actin filaments - red; cell nuclei - blue; viral antigen - green. Microscope magnification 60x, scale 20 μm