Fig. 10
Primary culture of murine neurons infected with MHV-JHM virus. Representative confocal images of neurons obtained at 2 (A,a’,a’’), 24 (B,b’,b’’), 48 (C,c’,c’’), 72 (D,d’,d’’), and 168 (E,e’,e’’) hours post infection. Green arrowheads point the presence of viral antigens in actin structures; Yellow arrowheads show areas of changes in β-tubulin resulting from MHV-JHM infection. White arrowheads point to syncytia formation. Purple arrowheads indicate cisternae microtubule structures. Yellow boxes indicate the overlapping presence of β-tubulin fluorescence with viral antigen. Indirect and direct immunofluorescence staining; merge images: β-tubulin - red; cell nuclei - blue; viral antigen - green. Microscope magnification 60x, scale 20 μm