Fig. 5
From: Endolysins of bacteriophage vB_Sal-S-S10 can naturally lyse Salmonella enteritidis
SDS-PAGE analysis of lysin1, holin, and lysin2. E. coli BL21 carrying recombinant plasmid (pCold-lysin1, pCold-holin, pCold-lysin2) was induced with 0.5 mM IPTG at 16 °C for 16 h. Ultrasonic purified proteins (left) and purified protein without TF tag (right) in the supernatant were detected by SDS–PAGE (full-length blots are presented in Fig. S5. The samples derive from the same experiment and blots were processed in parallel)