Optimisation of a double-centrifugation method for preparation of canine platelet-rich plasma

BMC Veterinary Research

Table 2 Platelet yield and recovery after the second centrifugation step

Run	Parameters			P value	Recovery (%)	P value
Run	RCF (× g)	Centrifugation time (min)	Yield (fold)	P value	Recovery (%)	P value
1	500	10	4.42 ± 0.84	0.0286^*	63.21 ± 4.66	0.0286^*
2	500	15	4.65 ± 0.76	0.0286^*	66.65 ± 3.64	0.0286^*
3	500	20	5.60 ± 0.49	0.1143	74.50 ± 1.51	0.0286^*
4	1000	10	5.41 ± 0.37	0.0286^*	72.06 ± 2.68	0.0286^*
5	1000	15	5.94 ± 0.36	0.3429	82.23 ± 6.51	0.4897
6	1000	20	5.65 ± 0.13	0.0286^*	78.08 ± 2.85	0.0286^*
7	1500	10	5.80 ± 0.48	0.1143	81.96 ± 2.58	0.0286^*
8	1500	15	6.48 ± 0.46	-	91.70 ± 4.49	-
9	1500	20	5.93 ± 0.28	0.3429	86.73 ± 1.54	0.4897
10	3000	10	5.82 ± 0.39	0.3429	85.06 ± 3.28	0.1143
11	3000	15	5.75 ± 0.49	0.3429	85.87 ± 1.65	0.0286^*
12	3000	20	4.34 ± 1.06	0.1143	64.79 ± 13.73	0.0286^*

Values expressed as mean ± standard deviation; RCF, relative centrifugal force. Asterisk (*) indicates a recovery or yield significantly different (P < .05) from condition 8, which produced the highest recovery and yield

ISSN: 1746-6148