Skip to main content

Table 4 Results of the PCR assay against a panel of mollicutes and non-mollicutes bacteria and their sensitivity, specificity, positive and negative predictive values

From: Comparison of different NAT assays for the detection of microorganisms belonging to the class Mollicutes

 

1a

2

3

4

5

6

Real-time

Mb

B

M

B

M

B

M

B

M

B

M

B

M

B

Test +

38

17

61

19

59

16

60

1

60

8

61

21

61

0

Test -

23

4

0

2

2

5

1

20

1

13

0

0

0

21

Sec

62.3%

(50.1-74.5)

100.0%

(100.0–100.0)

96.7%

(92.3–101.2)

98.4%

(95.2–101.5)

98.4%

(95.2–101.5)

100.0%

(100.0–100.0)

100.0%

(100.0–100.0)

Spc

19.0%

(2.3- 35.8)

9.5%

(−3.0–22.1)

23.8%

(5.6–42.0)

95.2%

(86.1–104.3)

61.9%

(41.1–82.7)

0.0%

(0.0–0.0)

100.0%

(100.0–100.0)

PPVc

69.1%

(56.9-81.3)

76.3%

(66.9–85.6)

78.7%

(69.4–87.9)

98.4%

(95.2–101.5)

88.2%

(80.6–95.9)

74.4%

(64.9–83.8)

100.0%

(100.0–100.0)

NPVc

14.8%

(1.4-28.2)

100.0%

(100.0–100.0)

91.5%

(85.4–97.5)

95.2%

(86.1–104.3)

92.9%

(79.4–106.3)

-

100.0%

(100.0–100.0)

  1. aPCR assays: 1, Hotzel et al. [17]; 2, Spergser et al. [27], 3, McAuliffe et al. [18]; 4, Van Kuppeveld et al. [6]; 5, Botes et al. [28]; 6, Ramírez et al. [16] and real-time PCR application of PCR assays n°4 and n°5
  2. bM: mollicutes, n: 61; B: non-mollicutes bacteria, n: 21
  3. cSe: Sensitivity; Sp: Specificity; PPV: Positive predictive value; NPV: Negative predictive value
Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us