Fig. 1

Construction of sptP deletion mutant in S. Enteritidis strain C50336. a PCR verification of C50336ΔsptP and C50336-Cm^R. The wild type strain harbors the complete sptP gene, with a PCR product length of 2509 bp, whereas the PCR product from C50336ΔsptP has a length of 1061 bp, and the product from the C50336-Cm^R has a length of 1991 bp (right). b Growth curves of wild-type S. Enteritidis C50336 and C50336ΔsptP. Bacteria were grown in liquid LB medium at 37 °C for 12 h with agitation, and the OD₆₀₀ values of triplicate cultures in LB medium were determined in 1-h intervals