A | ||||||
PCR | Agent | Real time PCR | ||||
R2 | Slope | Efficiency (%) | Linearity (ng) | Detection limit (ng/assay) | ||
Singleplex PCR | M. dispar | 0.9995 | −3.3836 | 97.49 | 10 ng-10 fg | 10 fg |
M. bovis | 0.9966 | −3.1137 | 109.49 | 10 ng-10 fg | 10 fg | |
M. bovirhinis | 0.9955 | −3.5033 | 92.95 | 10 ng-10 fg | 10 fg | |
Triplex PCR | M. dispar | 0.9989 | −3.1175 | 109.3 | 10 ng-10 fg | 10 fg |
M. bovis | 0.9955 | −3.6240 | 88.8 | 10 ng-100 fg | 100 fg | |
M. bovirhinis | 0.9939 | −3.3735 | 97.9 | 10 ng-100 fg | 100 fg | |
B | ||||||
PCR | Agent | Real time PCR | ||||
R2 | Slope | Efficiency (%) | Linearity (CFU/ml; log 10) | Detection limit (CFU/assay) | ||
Singleplex PCR | M. dispar | 0.995 | 3.248 ± 0.1276 | 103.2 | 6.5–2.5 | 1–2 |
M. bovis | 0.995 | 3.453 ± 0.1178 | 94.8 | 6.5–1.5 | 0.5 | |
M. bovirhinis | 0.981 | 3.395 ± 0.2698 | 97.0 | 5.5–1.5 | 0.5 | |
Triplex PCR | M. dispar | 1.000 | 3.534 ± 0.04608 | 91.9 | 6.5–2.5 | 1–2 |
M. bovis | 0.993 | 3.462 ± 0.1440 | 94.5 | 6.5–1.5 | 0.5 | |
M. bovirhinis | 0.965 | 2.750 ± 0.3014 | 131.0 | 5.5–1.5 | 0.5 |