Fig. 1

Cytotoxicity of GSNO and Hb on PK-15 cells tested by MTT assay. After incubation with the drugs for 72 h, MTT was added into each well. The cells were cultured for another 4 h, and 200 μl DMSO was added into each well for dissolving formazan, then A₅₇₀ of the samples were determined by a microplate reader. Relative viability was calculated according to the equation: Relative viability (%) = A₅₇₀ of the drug-treated sample / A₅₇₀ of the untreated sample × 100. Data shown were means ± SD from three independent experiments. a Cytotoxicity of GSNO on PK-15 cells. b Cytotoxicity of Hb on PK-15 cells. ^* P < 0.05, ^** P < 0.01 vs untreated control group