Table 1 Primer sequences used for PCR amplification and E. hepatopenaei (EHP) DIG probe preparation
Primer name | Sequence (5′-3′) | Amplicon (bp) | Reference |
|---|---|---|---|
SSU-PCR 1st PCR | Tangprasittipap et al. 2013 | ||
ENF779 ENR779 | CAGCAGGCGCGAAAATTGTCCA A AGAGATATTGTATTGCGCTTGCT | 779 | |
Nested PCR | |||
ENF176 ENR176 | CAACGCGGGAAAACTTACCA ACCTGTTATTGCCTTCTCCCTCC | 176 | |
EHP DIG probe | This paper | ||
ENF411 | AGGTGGTGTTAAAAGCCATTGAG | 235 | |
ENR176 | ACCTGTTATTGCCTTCTCCCTCC | ||