Table 2 PCR primers used for mRNA expression analysis

IL-2

F: 5′-CTGTATTTCGGTAGCAATG-3′

NM_204153.1

87-247

161

R: 5′-ACTCCTGGGTCTCAGTTG-3′

IL-1β

F: 5′-ACCCGCTTCATCTTCTACCG-3′

NM_204524.1

663-836

174

R: 5′-TCAGCGCCCACTTAGCTTG-3′

IL-6

F: 5′-GGCATTCTCATTTCCTTCT-3′

NM_204628.1

853-1051

199

R: 5′-CTGGCTGCTGGACATTTT-3′

IFN-α

F: 5′-GGACATGGCTCCCACACTAC-3′

XM_004937097.1

2896-3044

149

R: 5′-GGCTGCTGAGGATTTTGAAGA-3′

IFN-β

F: 5′-CACCACCACCTTCTCCT-3′

NM_001024836.1

66-321

256

R: 5′-TGTGCGGTCAATCCAGT-3′

CXCLi2

F: 5′-CATCATGAAGCATTCCATCT-3′

NM_205498.1

124-223

100

R: 5′-CTTCCAAGGGATCTTCATTT-3′

CXCLi1

F: 5′-AACTCCGATGCCAGTG-3′

NM_205018.1

176-380

205

R: 5′-TTGGTGTCTGCCTTGT-3′

CCR5

F: 5′-GTGGTCAACTGCAAAAAGCA-3′

NM_001045834.1

1126-1315

190

R: 5′-GCCCGTTCAACTGTGTCG-3′

MDA-5

F: 5′-GGACGACCACGATCTCTGTGT-3′

NM_001193638.1

457-535

79

R: 5′-CACCTGTCTGGTCTGCATGTTATC-3′

TLR3

F: 5′-GCTATTGAGCAAAGTCGAGA -3′

NM_001011691.3

2491-2693

203

R: 5′-ACAGGGGGCACTTTACTATT-3′

TLR7

F: 5′-TCTGGACTTCTCTAACAACA-3′

NM_001011688.2

1824-2010

187

R: 5′-AATCTCATTCTCATTCATCATCA-3′

TLR21

F: 5′-AGTTGTGTCCTGTGCTGAGAG-3′

NM_001030558.1

1035-1164

130

R: 5′-AGCAGGTTGTGTTCCACTGTC-3′

β-actin

F: 5′- AGACATCAGGGTGTGATGGTTGGT-3′

NM_205518.1

183-300

118

R: 5′-TGGTGACAATACCGTGTTCAATGG-3′