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Laboratory investigations into the origin of Mycoplasma synoviae isolated from a lesser flamingo (Phoeniconaias minor)

BMC Veterinary Research volume 12, Article number: 52 (2016) Cite this article

An Erratum to this article was published on 12 April 2016

Abstract

Background

The role of wild birds in the transmission and spread of mycoplasmas is not clear. Up to now different Mycoplasma species have been isolated from wild birds many of which are not considered pathogens sensu stricto for domestic flocks. This report describes the first isolation of Mycoplasma synoviae in a captive lesser flamingo (Phoeniconaias minor) held in a zoo in Italy and the laboratory investigations performed to elucidate its origin. Results showed that the strain was similar to the MS-H vaccine strain using the vlhA methods although no vaccination with this product was used in the zoo.

Case presentation

This paper describes investigations into a case in which 10 of 12 adult lesser flamingos (Phoeniconaias minor) died after having recently been moved from the Netherlands to a new zoo in Northern Italy. While most of the birds appeared to have died from the stress of movement and poor adaptation to their new environment, Mycoplasma synoviae, an important poultry pathogen in the layer and meat industry, was isolated for the first time from the trachea of one animal presenting catarrhal tracheitis and fibrinous airsacculitis. Genetic analysis of the conserved region of the vlhA was not able to differentiate the flamingo strain from the MS-H vaccine strain. However differences in the sequences of the obg gene of the flamingo and vaccine strain were detected. A test for temperature-sensitivity (ts) gave a ts − phenotype for the flamingo strain, in contrast to the ts + status of the MS-H strain. Based on this information and knowing that the flamingos were not vaccinated against M. synoviae, it is highly likely that the flamingo was infected with a genetically similar wild strain by contact with infected birds.

Conclusions

This case provides evidence for the potential role of international trade of ornamental birds as a possible route of introduction of new mycoplasma strains between countries, and moreover highlight that vlhA gene sequencing was not sufficient to discriminate the wild strain isolated from the flamingo from the MS-H vaccine strain.

Background

Mycoplasma synoviae, an important pathogen of poultry, can cause respiratory and articular disease, loss of production in the layer and meat industry [1]. In the poultry sector, control of mycoplasmosis is mainly based on the production and maintenance of mycoplasma-free breeders, with high levels of biosecurity measures applied to the flocks.

The role of wild birds in the transmission and spread of mycoplasmas is still unknown, despite the isolation of different Mycoplasma species from several avian species [2–8]. These include M. gallinaceum, M. iners, M. pullorum, M. columbinum, M. anatis, M. sturni, M. buteonis, M. falconis, M. gypis, M. corogypsi and M. neophronis; however none of these are considered pathogens sensu stricto for domestic flocks [9]. In contrast, some reports described infections of wild birds with pathogenic mycoplasmas of poultry, the most important of which is the infection of house finches (Carpodacus mexicanus) with M. gallisepticum in North America, causing a severe outbreak of conjunctivitis [10, 11]. However, the strain involved in these outbreaks was genetically different from vaccine, reference or field strains of M. gallisepticum isolated from commercial poultry [12].

In an epizootiological study of over 560 wild birds in Spain, M. synoviae was isolated from the tracheal and oropharynx samples of one red-legged partridge and three house sparrows [2]. Furthermore, M. meleagridis was isolated from captive falcons in the Middle East [13] and from free-ranging birds of prey in Europe [14]. In the latter case, infection could have arisen from a contaminated waste disposal site. Recently some authors have reported for the first time the isolation of M. iowae from the Eurasian stone-curlew (Burhinus oedicnemus) found in Gran Canaria [15]. More research is needed to understand the role of wild species in the maintenance and spreading of different strains of poultry pathogens species.

The lesser flamingo (Phoeniconaias minor) is a gregarious and social bird, very much appreciated by most ornithologists for its elegance. It is mainly distributed in Africa particularly in Tanzania, Kenya, Namibia, Botswana and South Africa, but there are also some breeding sites in south western and southern Asia. Several groups of this species are kept in captivity in the collection of some zoos worldwide [16]. Because of the particular reproductive behaviour of flamingos, breeding in captivity is difficult. In this report we describe the first isolation of M. synoviae from a captive lesser flamingo and investigate the variability of strains involved in the infection.

Case presentation

At the end of April 2010 a group of 12 adult lesser flamingos was moved from the Netherlands to a new zoo in northern Italy. These birds were kept in a large zoological garden enriched with a pond. The pond water was changed completely every week and the concrete surfaces were cleaned to avoid any growth of algae. The feeding program applied was considered appropriate for this species and birds received the same diet prescribed by the previous owner with the addition of a specific dietary supplement to supply birds during the critical and stressful phases.

The aviary in which the new birds were introduced already contained other flamingos but no aggressive behaviour or dominance interactions were seen. No other birds were present in the aviary. A few days after introduction, all new birds became sick, showing wet and ruffled feathers, hypomotility, lethargy, apathy, weakness, hypothermia and poor adaptation to the new environment. After 5 days some of the new birds started to die despite the administration of antibiotic treatment with enrofloxacin (100 mg/1 kg of moist food for 14 days) and mortality continued for some days. In total 10 out of 12 flamingos died showing similar clinical signs before death. These clinical signs and mortality are frequent findings in the acclimatizing phase of wild birds to captivity when this was considered common practice.

Materials and methods

Four carcasses of lesser flamingos were submitted to the Diagnostic Unit of Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro (PD, Italy) for necropsy examination and other diagnostic procedures. Several tissues (liver, intestine, lung, air sac and brain) were collected and submitted for bacteriological test; liver, intestine and foot pad lesion were collected for virological investigations and finally air sac was submitted for histopathological and mycological examinations.

The specimens were cultured for routine microbiological procedure both aerobically and anaerobically at 37 ° C. Tracheal swabs were collected from all carcasses and submitted for mycoplasma isolation using a selective broth medium for avian mycoplasmas (Mycoplasma Experience [ME], Reigate, UK) and incubated at 37 ° C under CO2 conditions for at least 15 days. During this time the cultures were checked daily and when a colour change or turbidity was seen, the broth was inoculated onto ME semi-solid medium, if no change was seen after 15 days, an aliquot of the broth was inoculated onto ME semi-solid medium (ME, Reigate, UK). The inoculated agar plates were checked for mycoplasmas daily for 15 days, after this period samples were considered negative. In order to identify the Mycoplasma species, DNAs were extracted from broths of suspect samples, and a 16S-rDNA PCR and denaturing gradient gel electrophoresis (DGGE) method was performed [17]. The results were also confirmed with a specific PCR for M. synoviae [18]. Moreover in order to confirm the purity of MS we performed the 16S rDNA identification. Briefly PCR products were cleaned-up using the Performa DTR Ultra 96-well kit (Edge BioSystems, Gaithersburg, MD). Treated PCR products were sequenced using BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA) in a 16-capillary ABI PRISM 3130xl Genetic Analyzer (Applied Biosystems, Foster City, CA). Sequence data were assembled and edited with SeqScape software v2.5 (Applied Biosystems).

In addition, the analysis of the variable lipoprotein haemagglutinin-A (vlhA) [19] and the sp0B-associated GTP binding (obg) [20] genes were applied to the M. synoviae isolates in order to detect intraspecific differences.

The following oligonucleotide primers were used in the study: obg-F (5’-GTT GAT AAA GGT GGA CCA G -3’), obg-R (5’-TTA GTG CAG ATA TCT CAA TG-3’) [21] and vlhaF (5’-ATT AGC AGC TAG TGC AGT GGC C-3’) and vlhaR2 (5’-AGT AAC CGA TCC GCT TAA TGC-3’) [19]. The positive amplified PCR products, obtained from both techniques, were then sequenced at the IZS (Venezie) where PCR products were purified with ExoSAP-IT (USB Corporation, Cleveland, OH) and sequenced in both directions using the Big Dye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA). The products of the sequencing reactions were cleaned-up using the Performa DTR Ultra 96-well kit (Edge BioSystems, Gaithers- burg, MD) and analyzed on a 16-capillary ABI PRISM 3130xl Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). Sequence data were assembled and edited with SeqScape software v2.5 (Applied Biosystems). Sequences obtained were submitted to GenBank®(NIH genetic sequence database) resulting respectively in HG421742.1 (vlhA gene) and KJ802785 (obg gene) accession numbers.

In order to compare the sequences with other Italian M. synoviae strains the MEGA 5.05 [22], Bioedit 7.0.0 [23] and BLAST search (http://www.ncbi.nlm.nih.gov/BLAST/) were used.

Once the strain of M. synoviae was identified, a differential growth at two different temperature (33°, and 39.5 °C) was performed in order to elucidate its temperature-sensitive phenotype (ts − or ts +). The capacity of the strain to replicate at different temperature was established using the colours changing unit (CCU) method on a 96-well plate, as described by others [24, 25]. The temperature-sensitive phenotype was attributed when a difference ≥103 CCU/ml occurs in the strain cultivated at the two different temperatures.

Moreover we decided to apply an additional test using three different temperatures (30°, 37° and 42 °C) in order to create larger differences between the lower and higher temperature of cultivation. The same test was performed with the MS-H vaccine strain in order to have a control sample.

Finally the vlhA and obg PCRs were applied also to the isolates grown at the three selected incubation temperatures (30°, 37° and 42 °C). All temperature-sensitive results were summarized in the Table 1.

Table 1 Temperature-sensitive results. In the table are reported the growth results at different temperature expressed in UCC/ml
Full size table

Discussion

Gross-pathology findings of the four birds included poor state of nutrition, absence of feed in the gastrointestinal tract and severe enteritis with some haemorrhagic intestinal areas. Only one bird (#2) showed catarrhal tracheitis and fibrinous airsacculitis (Fig. 1). All birds showed the same lesions such as chronic and septic lesions of the foot pads with joint enlargement, which were probably related to the bad conditions of the transport cage floor. Bacteriology results revealed pure cultures of Staphylococcus spp. (coagulase positive) from the air sac lesion as well as in the affected foot pad and Escherichia coli and Clostridium perfringens in the gut sample.

Fig. 1
figure 1

Gross pathology findings. Fibrinous airsacculitis in bird #2

Full size image

Mycological examination of the air sac revealed the presence of Candida spp. and Rhodotorula spp.. Moreover histopathology of the air sac showed several chronic inflammatory reactions with a necrotic centre surrounded by multinucleated giant cells (Fig. 2).

Fig. 2
figure 2

Histopathology findings. The cervical air sac is severely expanded by a focal nodular lesion composed of abundant heterophils partially surrounded by a single to double layer of macrophages and occasional multinucleated giant cells (heterophilic granuloma), the remaining tissue is diffusely enlarged by strong fibroplasia and moderate lymphoplasmacytic infiltration

Full size image

After only 24 h of incubation, the ME broth culture originating from the tracheal swab of bird #2 showed a colour change and 24 h later typical mycoplasma colonies were observed on the ME agar surface. M. synoviae was identified by three techniques: a specific M. synoviae PCR [18], a specific Mycoplasma DGGE [17] and by sequencing of the16S gene that confirmed only the presence of Mycoplasma synoviae. Figure 3 shows the same DGGE profile for the flamingo isolate as other M. synoviae field strains, as well as the positive controls including M. synoviae.

Fig. 3
figure 3

DGGE gel. In the lanes 1, 2 and 3 there are Mycoplasma gallisepticum, Mycoplasma synoviae and Mycoplasma meleagridis controls respectively. From lane 5 to 10 there are diagnostic samples positive for Mycoplasma synoviae: in lane 5 Mycoplasma synoviae NCTC (reference strain) and in lane 6 the strain isolated from flamingo (HG421742.1 IZSVE/2010/5711)

Full size image

After applying the PCR for vlhA gene to the isolate, a single amplicon was produced. This product was submitted for sequencing and the sequence obtained was compared with M. synoviae strains isolated in the previous year in the Italian geographical areas. The strain isolated from the flamingo did not match any Italian M. synoviae strains detected previously in our laboratory.

Based on the genotype classification of Bencina et al. [26] the strain was designated as genotype C subtype 3, whereas, according to the recent classification of Hammond et al., [19], the M. synoviae isolated from the flamingo represented a new group.

The BLAST search showed a similarity of 100 % with four M. synoviae strains, all isolated in Australia (GenBank® Accession no: DQ661614, AY913827, AY913822, AY913823) (Additional file 1).

Moreover the sequence obtained from the strain isolated from the lesser flamingo (IZSVe/2010/5711 GenBank® Accession no. HG421742.1) had a 100 % of similarity in the vlhA gene sequence with MS-H vaccine strain (AB501271) (Fig. 4, Additional file 1).

Fig. 4
figure 4

Phylogenetic analysis of the vlhA gene. Basing on the vlhA gene, the flamingo isolate (HG421742.1 IZSVE/2010/5711) clusters with the MS-H vaccine strain (AB501271) and the Australian isolate DQ661614. They are all classified as type C - subtype three by Bencina et al. [26] and group 13 by Hammond et al. [19]. The strains FN666087.1 and B91/96/798 are classified as type C3 - group 5; the strain B11/85 is a C1 – group 4. The strains FN666087.1, B91/96/798 and B11/85 have been introduced as examples of different genotypes to build the phylogenetic tree

Full size image

The temperature did not affect the variability in both vlhA and obg sequences (data not shown).

The BLAST analysis of the obg gene showed similarity of 99 % with M. synoviae strains isolated in Australia (GenBank® Accession no. KF875993.1, KF875997.1, KF875995, KF875994.19, [21]). Then nucleotide sequence was also compared to those investigated by Shahid et al. 2014 [21] (Additional file 2)

The obg gene of flamingo strain (IZSVe/2010/5711 GenBank® Accession no. KJ802785) differed from others in some nucleotide positions resulting in a different sequence from the MS-H and re-isolates (ts −, ts +) and parental strain [21]. Moreover in position 367 the flamingo obg sequence presented a G.

The temperature-sensitive test demonstrated a ts − phenotype because at 33 °C the titre was 1.8 × 104 CCU/ml, whereas at 39.5 °C the titre was 3.36 × 105 CCU/ml for the flamingo strain. In contrast the same test applied to the MS-H strain resulted in a titre of 1.8 × 108 CCU/ml for the lower temperature, while at 39.5 °C the titre was 1.27 × 102 CCU/ml. The additional test performed with a different range of temperature demonstrated a titre of 1.155 × 102 CCU/ml; 1.15 × 106 CCU/ml; 1.155 × 102 CCU/ml at 30, 37 and 42 °C respectively. Moreover the same test applied at MS-H vaccine strain showed a titre of 1.15 × 106 CCU/ml; 2.255 × 106 CCU/ml at 30 °C, 37 °C respectively, whereas there was no evidence of growth at 42 °C.

Conclusions

On the basis of gross pathology and histopathology findings it was concluded that the death of the birds was due to stress caused by movement and poor adaptation to their new captive conditions. The role of M. synoviae in this case, however, was not clear; indeed the air sac showed other opportunistic pathogens including fungi and bacteria such as Staphylococcus spp.. From the four tracheal samples, only one was positive for M. synoviae whereas the other three tracheal swabs were negative for Mycoplasma spp.. It is interesting to highlight that the positive sample were isolated from the trachea of the bird with fibrinous airsacculitis. Similar findings have been reported in birds of prey where bacteria and/or fungi were isolated simultaneously with M. meleagridis [14]. It is also interesting to point out that this strain of M. synoviae grew quickly (within 24 h in vitro) unlike most strains which take many days.

The significance of the flamingo strain depends on what genetic classification system is used for M. synoviae. Based on Bencina’s vlhA scheme [26] the strain was classified as the type C3, a strain which has never been detected in Italy before as far as we are aware. However, based on Hammond’s classification [19] this would represent the first isolation of a new genotype in the EU.

Regarding the identical vlhA gene sequence, we examined the possibility that the flamingo strain may have derived from the temperature-sensitive (ts +) M. synoviae vaccine strain MS-H by differential growth at different temperatures. This showed that the flamingo strain grew poorly at low temperature which strongly suggested that it is a wild strain. However there remains the possibility that a ts + strain could occasionally revert to a ts − MS-H strain [20, 27, 28]. This is highly unlikely given that there was no evidence that the flamingos had been vaccinated with the MS-H vaccine. Indeed during the period of isolation the use of MS-H within Europe, in particular, Italy, was not authorised. Finally the possibility of laboratory contamination can be excluded because strict quality control tests are performed daily.

Based on our data, the diagnostic application of the vlhA gene analysis is of limited value for the differentiation of field and vaccine strains (Fig. 4) as was previously proposed by Ogino et al. for Japanese strains [29], and has recently suggested for European strains by Dijkman et al. (2014) [30]. Shahid et al. [25] proposed a microtitration system coupled with real time PCR to distinguish the ts phenotype of M. synoviae because in Australia the vlhA sequencing is often inconclusive.

Moreover, investigating the obg sequence variability in the flamingo, different single nucleotide polymorphisms (SNPs) were detected, allowing to better distinguishing it from related MS-H strains. Analysis showed that the flamingo strain had a G in position 367, which based on previous results [21] classified it as a field strain and not related to the MS-H strain.

The majority of M. synoviae strains circulating in Italy which are non-vaccine wild type field strains can be identified by the vlhA method (unpublished data), while the microtitration system or the analysis of obg gene [21] could be used to distinguish the C-type strains which in Italy account for about 10 % of the rest.

Finally this clinical case provides good evidence for possible opportunistic transmission and spread of avian pathogens, which is compounded by the international trade in ornamental or zoo birds and may represent possible vectors of avian pathogens. These types of bird live in the open-air where strict biosecurity measures are not applied, thus enabling the direct and indirect contact with autochthonous fauna, increasing the risk of spreading infections if exotic microorganisms have contact with free populations. Hence these ornamental species could play an important epidemiological role in spreading infectious disease to the industrial poultry.

Abbreviations

DGGE:

Denaturing Gradient Gel Electrophoresis

DNA:

Deoxyribonucleic acid

EU:

European Union

ME:

Mycoplasma experience

MS:

Mycoplasma synoviae

obg:

spOB-associated GTP binding protein

PCR:

polymerase chain reaction

SNPs:

single nucleotide polimorphisms

ts:

temperature sensitive

vlhA:

variable lipoprotein hemagglutinin A

References

  1. Bradbury JM. Recovery of Mycoplasmas from Birds. In: Miles R, Nicholas R, editors. Mycoplasma Protocols. Totowa: Humana Press; 1998. p. 45–51.

    Chapter  Google Scholar 

  2. Poveda JB, Carranza J, Miranda A, Garrido A, Hermoso M, Fernandez A, Domenech J. An epizootiological study of avian mycoplasmas in southern Spain. Avian Pathology. 1990;19:627–33.

    Article  CAS  PubMed  Google Scholar 

  3. Poveda JB, Giebel J, Flossdorf J, Meier J, Kirchhoff H. Mycoplasma buteonis sp. nov., Mycoplasma falconis sp. nov., and Mycoplasma gypis sp. nov., Three Species from Birds of Prey. Int J Syst Bacteriol. 1994;44:94–8. doi:10.1099/00207713-44-1-94.

    Article  Google Scholar 

  4. Pennycott TW, Dare CM, Yavari CA, Bradbury JM. Mycoplasma sturni and Mycoplasma gallisepticum in wild birds in Scotland. Vet Rec. 2005;156:513–5.

    Article  CAS  PubMed  Google Scholar 

  5. Loria GR, Ferrantelli E, Giardina G, Li Vecchi L, Sparacino L, Oliveri F, McAuliffe L, Nicholas RA. Isolation and characterization of unusual Mycoplasma spp. from captive Eurasian Griffon (Gyps fulvus) in Sicily. J Wildl Dis. 2008;44:159–63.

    Article  CAS  PubMed  Google Scholar 

  6. Lierz M, Hagen N, Hernadez-Divers SJ, Hafez HM. Occurrence of mycoplasmas in free-ranging birds of prey in Germany. J Wildl Dis. 2008;44:845–50.

    Article  CAS  PubMed  Google Scholar 

  7. Stipkovits L, Szathmary S. Mycoplasma infection of ducks and geese. Poult Sci. 2012;91:2812–9.

    Article  CAS  PubMed  Google Scholar 

  8. Suárez-Pérez A, Ramírez AS, Rosales RS, Calabuig P, Poveda C, Rosselló-Móra R, Nicholas RA, Poveda JB. Mycoplasma neophronis sp. nov., isolated from upper respiratory tract of Canarian Egyptian Vulture (Neophron percnopterus majorensis). Int J Syst Evol Microbiol. 2012;62:1321–5.

    Article  PubMed  Google Scholar 

  9. Brown R.B. Phylum XVI. Tenericutes Murray 1984a, 356vp (Effective publication: Murray 1984b, 33). In: Bergey’s Manual of Systematic Bacteriology. 2nd Ed. Volume 4. New York Dordrecht Heidelberg London: Springer. p. 567–613.

  10. Ley DH, Berkhoff JE, McLaren JM. Mycoplasma gallisepticum isolated from house finches (Carpodacus mexicanus) with conjunctivitis. Avian Dis. 1996;40:480–3.

    Article  CAS  PubMed  Google Scholar 

  11. Fischer JR, Stallknecht DE, Luttrell P, Dhondt AA, Converse KA. Mycoplasmal conjunctivitis in wild songbirds: the spread of a new contagious disease in a mobile host population. Emerg Infect Dis. 1997;3:69–72.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Ley DH, Berkhoff JE, Levisohn S. Molecular epidemiologic investigations of Mycoplasma gallisepticum conjunctivitis in songbirds by random amplified polymorphic DNA analyses. Emerg Infect Dis. 1997;3:375–80.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Lierz M, Schmidt R, Runge M. Mycoplasma species isolated from falcons in the Middle East. Vet Rec. 2002;151:92–3.

    Article  CAS  PubMed  Google Scholar 

  14. Lierz M, Schmidt R, Brunnberg L, Runge M. Isolation of Mycoplasma meleagridis from free-ranging birds of prey in Germany. J Vet Med B Infect Dis Vet Public Health. 2000;47:63–7.

    Article  CAS  PubMed  Google Scholar 

  15. Suarez-Perez A, Ramirez AS, Rosales RS, Calabuig P, Poveda CG, Vega-Orellana OM, Mederos LE, Nicholas RAJ, Poveda JB. Isolation of Mycoplasma spp., from Eurasian Stone Curlew (Burhinus oedicnemus insularum) in Canary Island. Italy, Chianciano. In: Proceedings of the 18th International Congress of the International Organization for Mycoplasmology IOM. 2010. p. 107.

    Google Scholar 

  16. ISIS. International Species Information System. 2009. http://www2.isis.org/Pages/Home.aspx

  17. McAuliffe L, Ellis RJ, Lawes JR, Ayling RD, Nicholas RA. 16S rDNA PCR and denaturing gradient gel electrophoresis; a single generic test for detecting and differentiating Mycoplasma species. J Med Microbiol. 2005;54:731–9.

    Article  CAS  PubMed  Google Scholar 

  18. Lauerman LH, Hoerr FJ, Sharpton AR, Shah SM, Van Santen VL. Development and application of a polymerase chain reaction assay for Mycoplasma synoviae. Avian Dis. 1993;37:829–34.

    Article  CAS  PubMed  Google Scholar 

  19. Hammond PP, Ramírez AS, Morrow CJ, Bradbury JM. Development and evaluation of an improved diagnostic PCR for Mycoplasma synoviae using primers located in the haemagglutinin encoding gene vlhA and its value for strain typing. Vet Microbiol. 2009;136:61–8.

    Article  CAS  PubMed  Google Scholar 

  20. Shahid MA, Markham PF, Markham JF, Marenda MS, Noormohammadi AH. Mutations in GTP binding protein Obg of Mycoplasma synoviae vaccine strain MS-H: implications in temperature-sensitivity phenotype. PLoS One. 2013;8(9):e73954.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Shahid MA, Markham PF, Marenda MS, Agnew-Crumpton R, Noormohammadi AH. High-Resolution Melting-Curve Analysis of obg Gene to Differentiate the Temperature-Sensitive Mycoplasma synoviae Vaccine Strain MS-H from Non-Temperature-Sensitive Strains. PLoS One. 2014;9(3), e92215.

    Article  PubMed  PubMed Central  Google Scholar 

  22. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28:2731–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999;41:95–8. 1999 London University Press.

    CAS  Google Scholar 

  24. Morrow CJ, Markham JF, Whithear KG. Production of temperature-sensitive clones of Mycoplasma synoviae for evaluation as live vaccines. Avian Dis. 1998;42:667–70.

    Article  CAS  PubMed  Google Scholar 

  25. Shahid MA, Ghorashi SA, Agnew-Crumpton R, Markham PF, Marenda MS, Noormohammadi AH. Combination of differential growth at two different temperatures with a quantitative real-time polymerase chain reaction to determine temperature-sensitive phenotype of Mycoplasma synoviae. Avian Pathology. 2013;42:185–91.

    Article  CAS  PubMed  Google Scholar 

  26. Bencina D, Drobnic-Valic M, Horvat S, Narat M, Kleven SH, Dovc P. Molecular basis of the length variation in the N-terminal part of Mycoplasma synoviae hemagglutinin. FEMS Microbiol Lett. 2001;203:115–23.

    Article  CAS  PubMed  Google Scholar 

  27. Markham JF, Scott PC, Whithear KG. Field evaluation of the safety and efficacy of a temperature-sensitive Mycoplasma synoviae live vaccine. Avian Dis. 1998;42:682–9.

    Article  CAS  PubMed  Google Scholar 

  28. Noormohammadi AH, Jones JF, Harrigan KE, Whithear KG. Evaluation of the non-temperature-sensitive field clonal isolates of the Mycoplasma synoviae vaccine strain MS-H. Avian Dis. 2003;47:355–60.

    Article  PubMed  Google Scholar 

  29. Ogino S, Munakata Y, Ohashi S, Fukui M, Sakamoto H, Sekiya Y, Noormohammadi AH, Morrow CJ. Genotyping of Japanese field isolates of Mycoplasma synoviae and rapid molecular differentiation from the MS-H vaccine strain. Avian Dis. 2011;55:187–94.

    Article  PubMed  Google Scholar 

  30. Dijkman R, Feberwee A, Landman WJM. Variable lipoprotein haemagglutinin A (vlhA) gene sequence typing of mainly Dutch Mycoplasmma synoviae isolates: comparison with vlhA sequences from Genbank and with amplified fragment length polymorphism analysis. Avian Pathol. 2014;43(5):465–72.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We gratefully acknowledge the “Histopathological Laboratory” of the Istituto Zooprofilattico delle Venezie (Legnaro - Padova) who provided invaluable technical support.

This work was supported by the Italian Ministry of Health with the Project RC IZSVE 15/10 “Le micoplasmosi nel settore avicolo industriale: studio e messa a punto di nuove metodiche e protocolli diagnostici al fine di valutare e studiare il differente ruolo dei ceppi circolanti tra le differenti tipologie di produzioni avicole”

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Authors and Affiliations

  1. Istituto Zooprofilattico Sperimentale delle Venezie, viale dell’Universita’ 10, Legnaro, 35020, Padova, Italy

    Salvatore Catania, Federica Gobbo, Elisa Baldasso & Maria Luisa Moronato

  2. Università degli Studi di Padova, Dipartimento di Medicina Animale, Produzioni e Salute (MAPS), Legnaro, Padova, Italy

    Federica Gobbo & Maria Luisa Moronato

  3. Unidad de Epidemiología y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, C/Trasmontala s/n, Arucas, 35413, Islas Canarias, Spain

    Ana S. Ramirez

  4. Parco Faunistico LE CORNELLE, Valbrembo, Bergamo, Italy

    Davide Guadagnini

  5. Nutshell Lane, Farnham, Surrey, GU9 0HG, UK

    Robin A. J. Nicholas

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  1. Salvatore Catania
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  2. Federica Gobbo
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Correspondence to Salvatore Catania.

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Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

S-C carried out the necroscopies and diagnosis, management of the additional test perfomed; F-G carried out the necroscopies, the diagnosis, the isolation of Mycoplasma synoviae and the thermosusceptibility test; AS-R performed sequencing analysis; D-G managed the birds in the zoo and reported theanamnestic informations and the follow-up; E-B performed biomolecular tests and sequencing analysis, ML-M contributed to cultivation, biomolecular tests and sequencing analysis. RAJ-N supervised the activities and contributed to the draft. All authors read and approved the final manuscript.

Additional files

Additional file 1:

Alignment of partial vlhA sequences. Mycoplasma synoviae strains’ vlhA sequences showed a similarity with the lesser flamingo isolates (HG421742.1 IZSVE/2010/5711). In this figure we insert only one of the strains that showed a 100 % of similarity for the Australian isolate; its accession number is DQ661614 because the other one reported in the results showed the same sequence. The AB501271 is the vlhA sequence of MS-H vaccine strain. Finally, the accession number FN666087 is one of the two EU strains that showed the higher similarity. (TIF 1431 kb)

Additional file 2:

Alignment of partial obg sequences. The obg gene sequences of Mycoplasma synoviae isolated from flamingo (KJ802785 IZSVE/2010/5711) was aligned with MS-H (KC990837.1), re-isolate ts − (KC990838.1) and ts + (KC990839.1) and parental strain ts − (KC990840.1). Nucleotide differences are specified by the nucleotide, while dot represented no nucleotide changing. (TIF 608 kb)

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Catania, S., Gobbo, F., Ramirez, A.S. et al. Laboratory investigations into the origin of Mycoplasma synoviae isolated from a lesser flamingo (Phoeniconaias minor). BMC Vet Res 12, 52 (2016). https://doi.org/10.1186/s12917-016-0680-1

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BMC Veterinary Research

ISSN: 1746-6148

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