Fig. 6From: Construction of a camelid VHH yeast two-hybrid library and the selection of VHH against haemagglutinin-neuraminidase protein of the Newcastle disease virusValidation of VHH against cell derived HN protein. Pull down of cell derived HN protein from pCAGGS-HN transfected 293Â T cells lysate or pCAGGS empty vector transfected 293Â T cells lysate using VHH6 with talon metal affinity resin. Western blot with anti-HN polyclonal antibodies and anti-his tag mAbBack to article page