Fig. 1From: Construction of a camelid VHH yeast two-hybrid library and the selection of VHH against haemagglutinin-neuraminidase protein of the Newcastle disease virusInteraction between HN and VHH in yeast. The white clones in lane A showed that the bait(pBD-HN) has no autoactivator activity in yeast. When pBD-HN and 7 positive pAD-VHH were cotransformed into Y2HGold yeast strain, all cotransformants turned blue on DDO/X/A and QDO/X/A plates after incubation. At the same time, the pGBKT7-p53 and pGADT7-T co-transformants was used as positive control, whereas pGBKT7-Lam and pGADT7-T co-transformants was used as negative controlBack to article page