Fig. 5

Impact of LLL12 on colony formation in feline OSCC cell lines. a Feline OSCC cells were seeded at 1,000 cells per well in 6 –well plates for 24 h, followed by treatment with DMSO, 0.02, 0.2 or 2 μM LLL12 until formation of visible colonies. Cells were then fixed and stained with crystal violet and colonies greater than 50 cells were counted. After counting colonies, plating efficiency and survival fraction were calculated. Plating efficiency was defined as the number of colonies formed divided by the number of cells seeded in DMSO treated groups. Survival fraction was defined as the number of colonies formed divided by the number of cells seeded in LLL12 treated groups, normalized to the plating efficiency (*p < 0.0001). b Photographs of representative 6-well plates