Skip to main content

Table 2 Strain typing of 171 selected Staphylococcus aureus isolates from 2 herds 1

From: Effect of lactation therapy on Staphylococcus aureus transmission dynamics in two commercial dairy herds

Strain type

IMI5

Herd

In vitro Antimicrobial Susceptibility-Minimum Inhibitory Concentration values, MIC50/MIC90(ug/ml)6

antibiotic resistance PCR blaZ/mecA

PT2

ST3

MLST CC4

  

Amp

Ceph

Ceftio

Ery

Oxa

Pen

Pen: Novo

Pirl

Tet

 

1

151

705

3

2

≤0.12/0.25

≤2/≤2

≤0.5/1

≤0.25/≤0.25

≤2/≤2

≤0.12/≤0.12

≤(1:2)/≤(1:2)

≤0.5/1

≤1/≤1

−/−

 

2185

705

22

1

≤0.12/0.25

≤2/≤2

≤0.5/4

≤0.25/≤0.5

≤2/≤2

≤0.12/≤0.12

≤(1:2)/≤(1:2)

≤0.5/≤0.5

≤1/2

−/−

1a

2185

705

1

1

≤0.12

≤2

1

≤0.25

≤2

≤0.12

≤(1:2)

≤0.5

≤1

−/−

1b

2185

705

6

1

≤0.12/≤0.12

≤2/≤2

≤0.5/2

≤0.25/≤0.25

≤2/≤2

≤0.12/≤0.12

≤(1:2)/≤(1:2)

≤0.5/≤0.5

≤1/≤1

−/−

2185

705

3

1

≤0.12/≤0.12

≤2/≤2

1/1

≤0.25/≤0.25

≤2/≤2

≤0.12/≤0.12

≤(1:2)/≤(1:2)

≤0.5/≤0.5

≤1/≤1

−/−

1c

nd

 

1

1

≤0.12

≤2

1

≤0.25

≤2

≤0.12

≤(1:2)

≤0.5

4

−/−

2

2189

97

5

1

≤0.12/≤0.12

≤2/8

1/1

≤0.25/1

≤2/≤2

≤0.12/≤0.12

≤(1:2)/≤(1:2)

≤0.5/1

≤1/2

−/−

2189

97

7

1

≤0.12/≤0.12

≤2/≤2

1/1

≤0.25/≤0.25

≤2/≤2

≤0.12/≤0.12

≤(1:2)/≤(1:2)

≤0.5/1

≤1/≤1

−/−

2a

nd

 

1

1

≤0.12

≤2

2

≤0.25

≤2

≤0.12

≤(1:2)

2

4

−/−

2b

nd

 

4

1

≤0.12/≤0.12

≤2/≤2

1/1

≤0.25//≤0.25

≤2/≤2

≤0.12/≤0.12

≤(1:2)/≤(1:2)

≤0.5/2

2/4

−/−

3

8

5

7

2

0.5/0.5

≤2/≤2

1/1

≤0.25/≤0.25

≤2/≤2

0.25/0.5

≤(1:2)/≤(1:2)

≤0.5/≤0.5

≤1/≤1

+/−

4

352

97

2

2

≤0.12

≤2

2

≤0.25

≤2

≤0.12

≤(1:2)

≤0.5

≤1/≤1

−/−

4a

352

97

5

2

≤0.12/≤0.12

≤2/4

1/1

0.5/0.5

≤2/≤2

≤0.12/≤0.12

≤(1:2)/≤(1:2)

≤0.5/≤0.5

≤1/≤1

−/−

4bfh

nd

 

1

1

≤0.12

≤2

1

0.5

≤2

≤0.12

≤(1:2)

≤0.5

≤1/≤1

−/−

5

2187

97

1

1

≤0.12

≤2

1

≤0.25

≤2

≤0.12

≤(1:2)

≤0.5

≤1

−/−

5a

nd

 

1

1

≤0.12

≤2

1

≤0.25

≤2

≤0.12

≤(1:2)

≤0.5

≤1

−/−

6

nd

 

1

1

≤0.12

≤2

≤0.5

≤0.25

≤2

≤0.12

≤(1:2)

≤0.5

≤1

−/−

7

20

20

1

2

≤0.12

≤2

≤0.5

≤0.25

≤2

0.25

≤(1:2)

≤0.5

≤1

−/−

8

479

479

1

1

≤0.12

≤2

≤0.5

≤0.25

≤2

≤0.12

2/4

≤0.5

≤1

−/−

9†

87

59

0

1

8

4

2

8

4

8

≤(1:2)

1

2

+/+

10

25

15

1

3

16

≤2

1

≤0.25

≤2

8

≤(1:2)

≤0.5

≤1

+/−

11†

nd

 

0

2

≤0.25

≤2

≤0.5

≤0.25

≤2

0.25

≤(1:2)

≤0.5

≤1

−/−

  1. 1 Isolates from 74 IMI including 61 IMI identified in cows in treatment and control pens and13 IMI identified in cows that did not enter study pens. An additional 10 incidental isolates were also typed.
  2. 2 PFGE pulsotypes; types with the same number but different letters are minor variants with 1–3 band differences.
  3. 3 Sequence type determined by MLST.
  4. 4 MLST clonal complex predicted founder determined by eBurst MLST database (last searched May 30, 2012).
  5. 5 Number of quarters with IMI for each strain type.
  6. 6 Antimicrobial susceptibility for ampicillin (Amp), cephalothin (Ceph), ceftiofur (Ceftio), erythromycin (Ery), oxacillin (Oxa), penicillin (Pen), penicillin:novobiocin (Pen:Novo), pirlimycin (Pirl), and tetracycline (Tet); MIC50 and MIC90 values calculated as described in [25].
  7. † type found as only an incidental isolate, not defined as IMI or associated with subclinical mastitis (SCC< 200,000 cells/ml).
  8. fh type found in fresh heifer with clinical mastitis, cow did not enter study pens.
  9. nd = not determined.
Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us