Figure 5From: Generation and characterization of feline arterial and venous endothelial cell lines for the study of the vascular endotheliumSurface expression of cell adhesion molecules on EC lines in response to TNF-α. Immortalized aortic (A) or venous (B) endothelial cells were incubated for 6 h (E-selectin) or 12 h (ICAM-1 and VCAM-1) with either vehicle control or TNF-α at indicated doses. Surface expression was measured by ELISA as described in materials and methods. OD450, absorbance units at 450 nm. Bars show means with SD from 3 separate experiments. * Significantly different from control expression, P < 0.05.Back to article page