Identification of neglected cestode Taenia multicepsmicroRNAs by illumina sequencing and bioinformatic analysis

BMC Veterinary Research

Table 2 Six validated specific miRNAs

Candidate	Sequence	Count	Validated precursor
name
*tmu* -novel-4	CAACTCTAATGTCCCGGGTTA	381	GCAATTCCTCCATGTCCCTGAGACCCTACAGTTGTCCGGAATTTGCATATCCACATTTCTCACGTGGCAACTCTAATGTCCCGGGTTATGAGGTTGTG
*tmu* -novel-9	TTCGGAGTTTTGGGTGAGGT	18	TTTGCTTATTTTCGGAGTTCTGGGTGAGGTGTCGTTTTTCATGCACAGCTCAATCAACTCCGATAGATTTGTGGGCTGC
*tmu* -novel-10	GGCTGATTTGTGGGGCTCAGAA	54	CTGCGCAAGAGGCTGATTTGTGGGGCTCAGAAAAGCTGCAAATGAAAACTTTTGGTCCCCATTAACCAGCCGCCTGCAGA
*tmu* -novel-12	TCAGGACGCTACATTGGTAG	7	TTCTCTGTGTTAAATATATGTTCTGCCCCAGTACCATGCCTTCTTTTGCCTTGAAGGAAATCTCGGGACGCTACATTGGTAGCAGCGGAAAA
*tmu* -novel-19	TGATCACTGTGCCGGCGTATT	140	ACGGCAGAGGAGTATTTGGGTGAGGAGGTGACAAATGCGGTTGTCACAGTGCCGGAGTATTTGGGTGAGGAGGTGACAAATGCGGTTGTCACAGTGCCGGCGTATTTTAGTGTCAG
*tmu* -novel-20	TTTAGCTAGTAGAGAAGTGG	30	CCGGGTTCCATTTAGCTAGTAGAAAATTAGGGTAGGACATTGCAAACCACTTAATTCAAATCTTTATGCAGGAACAGTGGACAGCTCCGT

“Count” means the amount of the candidate novel miRNA. “Validated precursor” shows the corresponding precursor sequence obtained from genomic PCR validation.

ISSN: 1746-6148