Controlled, blinded force platform analysis of the effect of intraarticular injection of autologous adipose-derived mesenchymal stem cells associated to PRGF-Endoret in osteoarthritic dogs
© Vilar et al.; licensee BioMed Central Ltd. 2013
Received: 22 January 2013
Accepted: 24 June 2013
Published: 2 July 2013
Adipose-derived mesenchymal stem cell (ADMSC) therapy in regenerative medicine is a rapidly growing area of research and is currently also being used to treat osteoarthritis (OA). Force platform analysis has been consistently used to verify the efficacy of different therapeutic strategies for the treatment of OA in dogs, but never with AD-MSC.
The aim of this study was to use a force platform to measure the efficacy of intraarticular ADMSC administration for limb function improvement in dogs with severe OA.
Eight lame dogs with severe hip OA and a control group of 5 sound dogs were used for this study. Results were statistically analyzed to detect a significant increase in peak vertical force (PVF) and vertical impulse (VI) in treated dogs. Mean values of PVF and VI were significantly improved after treatment of the OA groups, reaching 53.02% and 14.84% of body weight, respectively, at day 180, compared with only 43.56% and 12.16% at day 0.
This study objectively demonstrated that intraarticular ADMSC therapy resulted in reduced lameness due to OA.
KeywordsOsteoarthrosis Hip Adipose-derived mesenchymal stem cells Force platform PRGF
Osteoarthritis (OA) is a common disorder in veterinary medicine, and clinicians are increasingly encountering this condition [1, 2]. However, restoration of the diseased articular cartilage in patients with OA is still a challenge for researchers and clinicians. Currently, several experimental strategies have investigated whether mesenchymal stem cells (MSCs), instead of chondrocytes, can be used for the regeneration and maintenance of articular cartilage in OA .
Autologous stem cell therapy in the field of regenerative veterinary medicine involves harvesting tissue,such as fat or bone marrow , from the patient, isolating the stem and regenerative cells, and administering the cells back to the patient . The field of adipose-derived MSC therapy (ADMSC) is a rapidly growing area of research, and it has been shown that stem cells have an affinity for damaged joint tissue; recent in vivo studies have confirmed that stem cells have the ability to localize and participate in the repair of damaged joint structures, including cruciate ligaments, menisci, and cartilage lesions . For these reasons, stem cell therapy is now being used to treat OA.
Previous studies evaluating OA therapy in dogs suggest that non-steroidal anti-inflammatory drugs do not often provide complete pain relief , perhaps because they rely on a single target receptor or pathway for their action. In contrast to drug therapy, cellular therapies such as ADMSCs play a trophic function by recruiting endogenous cells to the injured site. Studies and anecdotal clinical experience demonstrate that autologous ADMSC therapy is of clinical benefit in horses and dogs with orthopedic conditions [8–12].
Recent investigations have shown that growth factors contained in platelet-rich plasma (PRGF) act as vehicles and even potentiators of the effect of MSCs [13, 14]. The purpose of this study was to use force platform kinetic analysis to evaluate the effect of a single intraarticular injection of ADMSCs in 8 dogs with OA of hip joints by measuring peak vertical force (PVF) and vertical impulse (VI), which represent maximal weight bearing and distribution of forces through time, respectively.
Mean and standard deviation of PVF and VI in % dog weight (N/N and N.s/N, respectively) applied on the diseased leg
43.56 ± 0.73
49.21 ± 2.1
49.05 ± 1.51
53.2 ± 4.43
55.98 ± 0.62
53.96 ± 1.46
52.67 ± 1.91
51.53 ± 2.79
47.74 ± 1.34
47.94 ± 1.65
12.16 ± 0.72
13.71 ± 1.1
14.84 ± 1.29
16.09 ± 1.01
15.51 ± 1.28
15.12 ± 1.45
14.83 ± 1.54
14.51 ± 0.47
14.62 ± 0.41
14.67 ± 0.42
14.67 ± 0.51
Analysis of PVF
Compared with the control group, % PVF at D0 is significantly less (p-value< 0.001). In comparison, beyond this time differences became non significant (p-value >0.499).
Less-lame limbs analysis showed non significant differences till D30 (p value = 0.058); beyond D90, differences were significant (p-value<0.001).
Compared with control group, % PVF at D0 is significantly greater at D0 (p-value < 0.001), D30 (p-value =0.03) and D90 (p-value=0.015). At D180 this difference was not significant (p-value =0.14) (Figure 1).
Analysis of VI
Compared with the control group, % VI at D0 is significantly less (p-val = 0.024). In comparison, beyond this time differences became not significant (p-value > 0.84.
Less-lame limbs analysis showed no significant differences at any control period (p value > 0.462).
Compared with the control group, no significant differences were also found (p-values > 0.876) (Figure 2).
Association between more and less-lame limbs
The fitted linear mixed model shows a negative association between % PVF in less-lame limb and more-lame limb (β1<0, p-value=0.010). In % VI there is no association between VI in less and more -lame limb (β1=0, p-val=0.61).
The validity of the model fit was assessed by testing normality and homoscedasticity of the residuals. Both assumptions could be accepted: the Shapiro-Wilk test for normality and Levene’s test for homoscedasticity were not significant (P = 0.67 and P = 0.995, respectively).
In this trial, the effect of MSCs in lame OA dogs was investigated and quantified by means of an objective evaluation with a force platform. The ground reaction forces-related aspects of the gait, such as the PVF and VI, which represent maximal weight bearing and distribution of forces through time, respectively, measured the clinical impact of MSC treatment on the function of the limb during the stance phase of walking.
The absence of a direct relationship between radiographic evidence of OA and force platform findings is well known; in any case, in our study, diseased dogs were selected both on the basis of the presence or absence of radiographic evidence of severe OA (D-E degrees of hip dysplasia) and for evident lameness objectively determined by platform gait analysis .
Voss et al.  and Evans et al.  reported that force platform gait analysis at trot was much more sensitive than at walk for low-grade hindlimb lameness, but not for severe lameness. In our case, lameness of the OA dogs group was evident by direct observation, even at walk.
Although each dog had bilateral lameness, we believe that confident data could only be obtained from the more-lame limbs (lesser PVF), in order to limit a possible bias caused by inconsistent weight redistribution to the less affected contra-lateral hindlimb. In fact, mean values showed how initially less-lame limbs seemed to be “better” than limbs from the control group.
We observed a substantial improvement both in PVF and VI values through the period of evaluation, with an indicative gaining of limb function. Evolution of VI seems to be something controversial since some authors suggest that recordings of improvement in VI may suffer from a delay; their dogs tended to decrease their stance time in comparison with baseline values after non-steroidal anti-inflammatory drug treatment because they were able to improve their performance. However, other authors affirm that stance time did not change or increase when limb function improved [18–20]. In our study we observed independent evolution of both PVF and VI values, we hypothesize that this fact occurs because PVF only depends on the force exerted by the limbs, whilst VI reflects the evolution of the force during the whole support phase. For this reason VI could vary when one or both variables change (force and support time).
In 1999 Budsberg et al. compared the efficacy of a treatment(Etodolac) with a placebo control group and demonstrated a decrease in lameness in the treatment group using force platform analysis. In contrast, the degree of lameness in the dogs receiving the placebo deteriorated during the study period, making the control group unable to provide fixed reference data . Based on their results, we designed the experimental study using a control group of sound dogs that were able to provide fixed reference data.
Different adipose tissue donor sites have been found in revised literature: retroperitoneal adipose tissue , lateral thoracic area , gluteous fat  or inguinal region [5, 25]. We preferred this location to others because it is easier to access, abundant quantities of fat, absence of surgical complications and production of a non visible scar.
Regarding cartilage healing, Murphy et al. published in 2003 that the use of MSCs deposited in a fibrin matrix would be useful . However, although a recent equine study demonstrated early benefit, no significant differences were noted when MSCs plus fibrin were compared to fibrin alone at 8 months . Based on that work, it is likely that modulation of the matrix or cells will need to be accomplished to observe long-term benefit of MSCs for cartilage repair. Treatment timing in relation to the degree of pathology could also be a factor contributing to the insignificant results of the equine study. Specifically, because MSCs appear to have a tropism for damaged cells, including fibrillated articular cartilage, it may be that at day 14 (day of treatment) the degree of fibrillation was not enough to allow an MSC treatment effect. However, more observation and studies on cases with more advanced fibrillation need to be conducted to answer this question. Following those results, our study was designed using animals with severe and chronic OA, where chondral degeneration and fibrillation were clearly present. These criteria could explain why our diseased dogs showed highly significant improvements in limb function.
Moreover, our study supports previously published data demonstrating that a single intraarticular administration of ADMSCs associated to PRGF-Endoret decreases pain and lameness in dogs with OA over at least a 6-month period , although our study was conducted in a different joint and our results were supported by objective kinetic data.
A follow up of six months could be considered as a standard for testing the evolution of a medical or surgical treatment, although our dogs always seemed to improve during the first month after treatment. Beyond this time lapse not significant changes were statistically detected. Recent clinical evaluation of this same treatment in a much larger group of animals with osteoarthritis in hip or/and elbow joints are showing that apparently improvement could be prolonged for about 10 month (JM Carrillo, unpublished observations) when dogs seemed to start to worsen. This is a fact that could be contrasted with a biomechanic evaluation of a homogeneous larger group of hip OA dogs of the same breed and should encourage researchers to objectively determine when a new cycle of treatment should be useful to stop a relapse.
Regarding statistical analysis, more complex models could have been considered but we chose this one because it offers an adequate compromise between complexity and ability to represent the relationships between the considered variables [28, 29].
Force platform analysis demonstrated quantitatively that ADMSC+ PRGF-Endoret therapy shows significant potential for clinical use in the treatment of lameness associated with OA.
Although this study was limited to a small number of dogs with severe OA, MSC therapy was found to be an appropriate treatment for hip joints, in terms of its efficacy in objectively improving the dogs’ gait and ability to live a more normal life, and the absence of side effects. In the future, ADMSCs could also become a promising therapeutic strategy in human OA.
The research protocol was revised and authorized by the Ethical Committee of Animal Welfare (CEBA) of the University of Las Palmas de Gran Canaria (Spain) with reference code: 001/2010 CEBAULPGC.
Eight adult client-owned Canarian Presa dogs (5 males, 3 females) with lameness and pain attributed to OA associated with hip dysplasia were included in the study. The dogs were affected by chronic OA and had not received any kind of medications (e.g., non-steroidal anti-inflammatory drugs, analgesics), nutraceuticals (e.g., glucosamine or chondroitin, vitamin E, omega-3 fish oil), or adjunctive therapies (e.g., acupuncture) for at least 2 months. A control group consisted of 5 sound and healthy dogs of the same breed.
None of the dogs were forced to perform physical activity. Dog owners were informed and granted a signed consent for the whole procedure.
Radiographs confirmed the presence of OA compatible with D and E degrees of hip dysplasia as defined by the Fédération Cynologique Internationale (World Canine Organization). D- degree dysplasic dogs showed obvious deviation from the normal with evidence of a shallow acetabulum, flattened femoral head, poor joint congruency, and in some cases, subluxation with marked changes of the femoral head and neck. E-degree dysplasic dogs showed complete dislocation of the hip and severe flattening of the acetabulum and femoral head . Additional radiographs of knee and elbow joints were taken after physical and orthopedic examinations were performed to ensure that hip OA was the main reason for the observed clinical signs and that general health was otherwise normal.
Extraction and culture-inoculation of ADMSCs and PRGF-Endoret
Stem cell extraction and inoculation phases were performed under general anesthesia (sevofluorane). Adipose tissue (20 g) was collected from the inguinal region through a small surgical incision and then included in a sterile bottle with culture medium for the cellular maintenance. Additionally, 120 ml of blood was extracted in sterilized conditions and deposited in 8 ml serum tubes. The adipose tissue and the blood tubes were sent to Fat-Stem Laboratories (Buggenhout, Belgium) where they were processed using the patent protocol of Dog Stem® (Fat-Stem). The number of messenchymal stem cells was over 30 million, shown in the laboratory certificate for the quality of the cells and were sent in two 2 ml tubes with 15 million per tube.
Once the AMSC were received they were infiltrated with the PRGF-Endoret that was prepared in that moment following a patented method : 20 ml of blood were aseptically collected in four 4.5 ml citrate tubes, then centrifugated during 8 minutes at 460 G. Before the infiltration the PRGF was activated with 5% of its volume with 10% calcium chloride.
Subsequently, PRGF-Endoret was associated to ADMSC. The resultant 4 ml solution was injected aseptically into the hip joints through conventional arthrocentesis sites.
The needle was introduced just cranioproximal to the trochanter major, aimed slightly ventrally and caudally. The appearance of joint fluid confirmed proper needle placement .
Gait analysis was performed using a single platform mounted in the center of, and level with, a 7-m runway covered by a rubber mat. The mat weight was discarded setting to “0 force” with the tare button after the platform was covered. Dogs were leash guided at walk over the force platform by the same handler. Walk velocity was measured by use of a motion sensor (Pasco, California, USA) positioned 1 m from the platform.
Five valid trials, at a sampling frequency of 250 Hz, were obtained for each dog. A trial was considered valid when the limb fully contacted the force platform, and with the dog walking next to the handler without pulling on the leash. The trial was discarded if the dog was distracted during the measurement, if the limb struck the edge of the force plate, or if any portion of the contralateral paw hit the force plate. A member of the research team (BC) evaluated the trial to confirm which limb touched the center of the force platform.
The platform was interfaced with a dedicated computer using DataStudio (Pasco, California, USA), software specially designed for the acquisition, numerical conversion, and storage of data. A team member (JMV) recorded data from both affected limbs at day 0, 30, 90, and 180 post-treatment; the obtained PVF and VI values were normalized relative to body weight (%) to characterize the possible improvement of lameness during treatment with MSCs.
Significance of the differences in PVF and VI between periods of observation were tested by means of analysis of variance of these models. Following this analysis, post-hoc comparisons between fixed effects were performed using Tukey’s procedure. For assessing the validity of the model, the Shapiro-Wilk test was applied for testing normality of the residuals.
Here yij represents the value (PVF or VI) in the less-lame limb and xij the value in the more-lame limb.
Significance level was set at P ≤ 0.05 in all tests.
Adipose-derived mesenchymal stem cells
Mesenchymal stem cells
Plasma rich in growth factors
Peak vertical force
This work was supported by the Fundación García-Cugat and the Veterinary Clinic Hospital of the Universidad de Las Palmas de Gran Canaria. We also acknowledge Misty Bailey for technical editing.
- Bockstahler B, Krautler C, Holler P, Kotschwar A, Vobornik A, Peham C: Pelvic limb kinematics and surface electromyography of the vastus lateralis, biceps femoris, and gluteus medius muscle in dogs with hip osteoarthritis. Vet Surg. 2012, 41: 54-62.PubMedView ArticleGoogle Scholar
- Silvestre AM, Ginja MM, Ferreira AJ, Colaço J: Comparison of estimates of hip dysplasia genetic parameters in Estrela Mountain dog using linear and threshold models. J Anim Sci. 2007, 85: 1880-1884. 10.2527/jas.2007-0166.PubMedView ArticleGoogle Scholar
- Oldershaw R: Cell sources for the regeneration of articular cartilage: the past, the horizon and the future. Int Exp Path. 2012, 93: 389-400.Google Scholar
- Takemitsu H, Zhao D, Yamamoto I, Harada Y, Michishita M, Arai T: Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells. BMC Vet Res. 2012, 8: 150-10.1186/1746-6148-8-150.PubMedPubMed CentralView ArticleGoogle Scholar
- Guercio A, Di Marco P, Casella S, Cannella V, Russotto L, Purpari G, Di Bella S, Piccione G: Production of canine mesenchymal stem cells from adipose tissue and their application in dogs with chronic osteoarthritis of the humeroradial joints. Cell Biol Int. 2012, 36: 189-94. 10.1042/CBI20110304.PubMedView ArticleGoogle Scholar
- Agung M, Ochi M, Yanada S, Adachi N, Izuta Y, Yamasaki T, Toda K: Mobilization of bone marrow-derived mesenchymal stem cells into theinjured tissues after intraarticular injection and their contribution to tissueregeneration. Knee Surg Sports Traumatol Arthrosc. 2006, 14: 1307-1314. 10.1007/s00167-006-0124-8.PubMedView ArticleGoogle Scholar
- Vilar JM, Morales M, Santana A, Batista M, Mirò F, Spinella G: Long-term valuation of oral mavacoxib in osteoarthrosic dogs using force platform analysis. Pak Vet J. 2013, 33: 229-233.Google Scholar
- Harman R, Cowles B, Orava C: A retrospective review of 62 cases of suspensory ligament injury insport horses treated with adipose-derived stem and regenerative cell therapy. Proceedings of the Veterinary Orthopedic Society. Edited by: Palmer R. 2006, Keystone, 212-214.Google Scholar
- Dahlgren LA: Use of adipose derived stem cells in tendon and ligament injuries. Proceedings of the American College of Veterinary Surgeons Symposium Equine and Small Animal. Edited by: Schneider RK. 2006, Washington, 150-151.Google Scholar
- Nixon A, Dahlgren L, Haupt J, Yeager AE, Ward DL: Effect of adipose-derived nucleated cell fractions on tendon repair in horses with collagenase-induced tendinitis. Am J Vet Res. 2008, 69: 928-937. 10.2460/ajvr.69.7.928.PubMedView ArticleGoogle Scholar
- Black LL, Gaynor J, Gahring D, Adams C, Aron D, Harman S, Gingerich DA, Harman R: Effect of adipose-derived mesenchymal stem and regenerative cells on lameness in dogs with chronic osteoarthritis of the coxofemoral joints: a randomized, double-blinded, multicenter, controlled trial. Vet Ther. 2007, 8: 272-84.PubMedGoogle Scholar
- Black LL, Gaynor J, Adams C, Dhupa S, Sams AE, Taylor R, Harman S, Gingerich DA, Harman R: Effect of intraarticular injection of autologous adipose-derived mesenchymal stem and regenerative cells on clinical signs of chronic osteoarthritis of the elbow joint in dogs. Vet Ther. 2008, 9: 192-200.PubMedGoogle Scholar
- Ball SG, Shuttleworth A, Kielty CM: Inhibition of platelet-derived growth factor receptor signaling regulates Oct4 and Nanog expression, cell shape, and mesenchymal stem cell potency. Stem Cells. 2012, 30: 548-60. 10.1002/stem.1015.PubMedPubMed CentralView ArticleGoogle Scholar
- Rodríguez FJ, Valdés T, Carrillo JM, Rubio M, Monleon M, García DM, García M, Cugat R, Moreno V: Platelet-rich plasma favors proliferation of canine adipose-derived mesenchymal stem cells in methacrylate-endcapped caprolactone porous scaffold niches. J Funct Biomater. 2012, 3: 556-568. 10.3390/jfb3030556.View ArticleGoogle Scholar
- Gordon WJ, Conzemius MG, Riedesel E, Besancon MF, Evans R, Wilke V, Ritter MJ: The relationship betweenlimb function and radiographic osteoarthrosis in dogs with stifle osteoarthrosis. Vet Surg. 2003, 32: 451-454. 10.1053/jvet.2003.50051.PubMedView ArticleGoogle Scholar
- Voss K, Imhof J, Kaestner S, Montavon PM: Force plate gaitanalysis at the walk and trot in dogs with low-grade hindlimb lameness. Vet Comp OrthopTraumatol. 2007, 20: 299-304.Google Scholar
- Evans R, Gordon W, Conzemius M: Effect of velocity on ground reaction forces in dogs with lameness attributable to tearing of the cranial cruciate ligament. Am J Vet Res. 2003, 64: 1479-1481. 10.2460/ajvr.2003.64.1479.PubMedView ArticleGoogle Scholar
- Moreau M, Lussier B, Doucet M, Vincent G, Martel-Pelletier J, Pelletier JP: Efficacy of licofelone in dogs with clinical osteoarthritis. Vet Rec. 2007, 160: 584-588. 10.1136/vr.160.17.584.PubMedView ArticleGoogle Scholar
- Bennett RL, DeCamp CE, Flo GL, Hauptman JG, Stajich M: Kinematic gait analysis in dogs with hip dysplasia. Am J Vet Res. 1996, 57: 966-71.PubMedGoogle Scholar
- DeCamp CE, Riggs CM, Olivier NB, Hauptman JG, Hottinger HA, Soutas-Little RW: Kinematic evaluation of gait in dogs with cranial cruciate ligament rupture. Am J Vet Res. 1996, 57: 120-126.PubMedGoogle Scholar
- Budsberg SC, Johnston SA, Schwarz PD, DeCamp CE, Claxton R: Efficacy of etodolac for the treatment of osteoarthritis of the hip joints in dogs. J Am Vet Med Assoc. 1999, 214: 206-210.PubMedGoogle Scholar
- Bigham-Sadegh A, Mirshokraei P, Karimi I, Oryan A, Aparviz A, Shafiei-Sarvestani Z: Effects of adipose tissue stem cell concurrent with greater omentum on experimental long-bone healing in dog. Connect Tissue Res. 2012, 53: 334-342. 10.3109/03008207.2012.660585.PubMedView ArticleGoogle Scholar
- Haghighat A, Akhavan A, Hashemi-Beni B, Deihimi P, Yadegari A, Heidari F: Adipose derived stem cells for treatment of mandibular bone defects: An autologous study in dogs. Dent Res J (Isfahan). 2011, 8 (1): 51-57.Google Scholar
- Kang BJ, Ryu HH, Park SS, Koyama Y, Kikuchi M, Woo HM, Kim WH, Kweon OK: Comparing the osteogenic potential of canine mesenchymal cells derived from adipose tissues, bone marrow, umbilical cord blood, and wharton’s jelly for treating bone defects. J Vet Sci. 2012, 13: 299-310. 10.4142/jvs.2012.13.3.299.PubMedPubMed CentralView ArticleGoogle Scholar
- Oh HJ, Park EJ, Lee SY, Soh JW, Kong IS, Choi SW, Ra JC, Kang SK, Lee BC: Comparison of cell proliferation and epigenetic modification of gene expression patterns in canine foetal fibroblasts and adipose tissue-derived mesenchymal stem cells. Cell Prolif. 2012, 45: 438-444. 10.1111/j.1365-2184.2012.00838.x.PubMedView ArticleGoogle Scholar
- Murphy JM, Fink DJ, Hunziker EB, Barry FP: Stem cell therapy in a caprine model of osteoarthritis. Arthritis Rheum. 2003, 48: 3464-3474. 10.1002/art.11365.PubMedView ArticleGoogle Scholar
- Wilke MM, Nydam DV, Nixon AJ: Enhanced early chondrogenesis inarticular defects following arthroscopic mesenchymal stem cell implantation in anequine model. J Orthop Res. 2007, 25: 913-925. 10.1002/jor.20382. inki, Finland. Mimeograph, p. 25PubMedView ArticleGoogle Scholar
- Pinheiro J, Bates D: Fitting linear mixed-effects models. Mixed-effects models in S and S-PLUS. Edited by: Chambers J. 2000, New York: Springer-Verlag, 133-197. 1View ArticleGoogle Scholar
- Milliken GA, Johnson DE: Analysis of mixed models/case studies of a mixed model. Analysis of messy data. 2009, Florida: Chapman and Hall, 385-417. 1, 2View ArticleGoogle Scholar
- Piermattei D, Flo G, DeCamp C: Hip joint. Brinker, Piermattei and Flo’s handbook of small orthopedics and fracture repair. Edited by: Fathman L. 2006, Missoury: Saunders, 461-511. 4Google Scholar
- Anitua E, Sánchez M, Orive G, Andía I: The potential impact of the preparation rich in growth factors (PRGF) in different medical fields. Biomaterials. 2007, 28: 4551-4560. 10.1016/j.biomaterials.2007.06.037.PubMedView ArticleGoogle Scholar
- Piermattei D, Flo G, DeCamp C: Orthopedic examination and diagnostic tools. Brinker, Piermattei and Flo’s handbook of small orthopedics and fracture repair. Edited by: Fathman L. 2006, Missoury: Saunders, 3-24. 4Google Scholar
- R Development Core Team: R: A language and environment for statistical computing. http://www.R-project.org/,
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