Figure 1

Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis of the gut bacterial community after different treatments. PCR-DGGE of the 16 S rDNA V3 region was performed to analyze the bacterial community. Similarities were assessed by cluster analysis using Quantity One. (A) Nine fecal samples were randomly collected at the beginning of the experiment. (B, C, and D) Fecal, cecal, and ileal samples collected at the end of the experiment. B1–B3, B4–B6, and B7–B9 represent fecal samples collected from groups 1, 2-1, and 2-2, respectively. C1–C3, C4–C6, and C7–C9 represent cecal contents collected from groups 1, 2-1, and 2-2, respectively. D1–D3, D4–D6, and D7–D9 represent ileal mucosa collected from groups 1, 2-1, and 2-2, respectively. In Group 1, mequindox was added to the subjects’ diet. In Group 2-1, L. plantarum ZJ316 was added and mequindox was removed, and samples were collected immediately after halting the addition of L. plantarum ZJ316 (day 95). In Group 2-2, L. plantarum ZJ316 was added and mequindox was removed, and samples were collected one week after the cessation of addition of L. plantarum ZJ316 (day 102).