Figure 4

Evaluation of STAT3-related gene expression using qRT-PCR after LLL12 treatment. Canine OS cell lines were treated with DMSO or 0.5 μM LLL12 for 12 hours. RNA was collected and qRT-PCR was performed for survivin, cyclin D1, BCL-2 and MCL-1 and 18 s. Experiments were performed in triplicate and repeated three times. Delta CTs from qRT-PCR were compared using two sample t-tests and Holm’s method was used to control type-I error across tests of multiple genes (**p<0.02, *p<0.001).