Development and application of a SYBR green RT-PCR for first line screening and quantification of porcine sapovirus infection

Table 2 Specificity of the SYBR Green qRT-PCR based on melting curve temperature analysis

Infecting virus	Genogroup and genotype*	Identifier	Melting temperature
PoSaV	III	PC33	87.0
PoNoV	II	PC54	83.5
		PC55	83.5
		PC58	83.5
		PC59	83.5
		PC60	83.5
		PC61	83.5
HuNoV	I.4	Hu360	83.5
	II	HuJ	83.5
	II.2	Hu333	83.5
	II.4	Hu384	83.5
	II.4	Hu498	83.5
BoNoV	III.1	BV164	83.5
	Recombinant III.1/III.2	BV416	84.0
	III.2	BV52	83.5
	III.2	BV15	83.5
	III.2	BV253	83.5
	III.2	BV344	83.0
FCV	ND	F106	84.0
		F117	83.5
		F119	84.0
		F226	84.0
		F242	83.5
		F295	83.0

* Genogrouping and genotyping are based on phylogenetic relationships in polymerase sequence. PoSaV: porcine sapovirus; HuSaV: human sapovirus; PoNoV: porcine norovirus; HuNoV: human norovirus; BoNoV: bovine norovirus; FCV: feline calicivirus; BoKoV: bovine kobuvirus; ND: not done.

ISSN: 1746-6148