Figure 7From: A role of ghrelin in canine mammary carcinoma cells proliferation, apoptosis and migrationGhrelin affects viability of canine mammary carcinoma cells. The canine mammary cancer cells viability determined by MTT assay in control conditions (ctrl) and in a presence of n-octanoylated ghrelin peptide (Peptides International) at concentrations of 1, 10, 100 and 1000 nM for 24 h. Absorbance at 570 nm has been converted to percentages above control (±SEM). The experiment has been conducted in n = 15 repetitions. Ghrelin treatment at the concentration of 1 and 10 nM; 1 and 100 nM, 1-1000 nM significantly increased cell viability of the CMT-W1 (A), CMT-W1M (B) and CMT-W2 (C) cell lines, respectively. No significant differences has been observed in CMT-W2M cell line (D). The statistical analysis was performed using Prism version 5.00 software (GraphPad Software, USA). One-way ANOVA followed by Dunnett’s post hoc comparisons were applied. p < 0.05 was marked as *, p < 0.01 was marked as ** and p < 0.001 was marked as ***.Back to article page