Table 2 The primers used to create the various truncated, single and multiple mutants by PCR in this study

VP2 111 N del EcoRI

Forward

26-mer

TGGAATTCATGGAGGACCGATCAACC

VP2 141 N del EcoRI

Forward

26-mer

AGGAATTCATGCACTACTCCCAGCCG

VP2 160 N del EcoRI

Forward

26-mer

AGGAATTCATGGACGAGCTCGCAGAC

VP2 115 C del XhoI

Reverse

24-mer

TCCTCGAGTGATCGGTCCTCAAGT

VP2 132 C del XhoI

Reverse

23-mer

TCCTCGAGACCCTGTACTCGGAG

VP2 145 C del XhoI

Reverse

26-mer

TCCTCGAGCTGGGAGTAGTGGTAATC

VP2 136-138A

Forward

27-mer

AAACGAGCTGCTGCTGCTCTTGATTAC

VP2 136-138A

Reverse

27-mer

GTAATCAAGAGCAGCAGCAGCTCGTTT

VP2 150-152A

Forward

39-mer

ACCCCGAACGCAGCAGCAGTGTATAAGACTGTAAGATGG

VP2 150-152A

Reverse

39-mer

CCATCTTACAGTCTTATACACTGCTGCTGCGTTCGGGGT

VP2 136-138A/134/A

Forward

27-mer

GTACAGGGTAAAGCTGCTGCTGCTGCT

VP2 136-138A/134/A

Reverse

27-mer

AGCAGCAGCAGCAGCTTTACCCTGTAC

VP2 136-138A/133/A

Forward

27-mer

GTACAGGGTGCTCGAGCTGCTGCTGCT

VP2 136-138A/133/A

Reverse

27-mer

AGCAGCAGCAGCTCGAGCACCCTGTAC

VP2136-138A/133A/134/A

Forward

27-mer

GTACAGGGTGCTGCTGCTGCTGCTGCT

VP2 136-138A/133A/134/A

Reverse

27-mer

AGCAGCAGCAGCAGCAGCACCCTGTAC

VP2 133A

Forward

28-mer

GTACAGGGTGCTCGAGCTAAAAGAAAGC

VP2 133A

Reverse

28-mer

GCTTTCTTTTAGCTCGAGCACCCTGTAC

VP2 134A

Forward

28-mer

GTACAGGGTAAAGCTGCTAAAAGAAAGC

VP2 134A

Reverse

28-mer

GCTTTCTTTTAGCAGCTTTACCCTGTAC

VP2 133A/134A

Forward

28-mer

GTACAGGGTGCTGCTGCTAAAAGAAAGC

VP2 133A/134A

Reverse

28-mer

GCTTTCTTTTAGCAGCAGCACCCTGTAC

VP2 C95S

Forward

20-mer

CGCTAAGATCAGCAACTGCG

VP2 C95S

Reverse

22-mer

CGCAGTTGCTGATCTTAGCGTG

VP2 C97S

Forward

21-mer

ATCTGCAACAGCGGACAATTC

VP2 C97S

Reverse

24-mer

ATTGTCCGCTGTTGCAGATCTTAG

VP2 C95S/C97S

Forward

28-mer

CGCTAAGATCAGCAACAGCGGACAATTC

VP2 C95S/C97S

Reverse

28-mer

ATTGTCCGCTGTTGCTGATCTTAGCGTG