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Figure 3 | BMC Veterinary Research

Figure 3

From: Identification of the NLS and NES motifs of VP2 from chicken anemia virus and the interaction of VP2 with mini-chromosome maintenance protein 3

Figure 3

Mutant constructs of VP2-GFP that were used in this study. (A) Truncated fragments of the VP2 (black bars) encoding constructs fused with GFP (white bars) were used in this study. The right panels indicate the localization patterns in cells. GFP distribution in the cytosol is indicated by C; GFP signal in nucleus that is stronger than that in the cytosol is indicated by N; GFP detected in both the cytosol and nucleus is indicated by N/C. The putative NLS motifs (BiNLS1 and NLS2) and the weak NES motif are shown. The amino acids present in the constructs are also indicated. (B) Subcellular localization of C-terminal deleted VP2-GFP constructs (115dC, 132dC and 145dC) in HeLa and CHO cells (green). (C) Subcellular localization of N-terminal deleted VP2-GFP constructs (111dN, 141dN and 160dN) in HeLa and CHO cells (green). All cells were fixed and stained with DAPI (blue)

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BMC Veterinary Research

ISSN: 1746-6148

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