Table 1 Pathogens used and RT-LAMP assay results
Source | RT-LAMP result | |||
|---|---|---|---|---|
Agarose gel electrophoresis | Color change after adding dye | |||
Bovine rotavirus (BRV) NCDV | genotype G6P[1] | CVCC | + | + |
BRV014 | G6P[5] | CVCC | + | + |
GX-BRV-1 | G6P[11] | GVRI | + | + |
GX-BRV-2 | G6P[5] | GVRI | + | + |
GX-BRV-3 | G10P[11] | GVRI | + | + |
GX-BRV-4 | G6P[11] | GVRI | + | + |
GX-BRV-5 | G6P[11] | GVRI | + | + |
GX-BRV-6 | G6P[5] | GVRI | + | + |
GX-BRV-7 | G6P[5] | GVRI | + | + |
GX-BRV-8 | G10P[11] | GVRI | + | + |
Other bovine pathogens | ||||
Bovine virus diarrhea (BVDV) | CVCC | - | - | |
Mycobacterium bovis (MB) | GVRI | - | - | |
Classical swine fever virus (CSFV) | CVCC | - | - | |
Infective bovine rhinotracheitis virus (IBRV) | CVCC | - | - | |
Bovine Coronavirus (BCV) | GVRI | - | - | |
Negative control | ||||
Rectal swab of normal bovine1 | GVRI | - | - | |
Rectal swab of normal bovine2 | GVRI | - | - | |
Rectal swab of normal bovine3 | GVRI | - | - | |
Nasal swab of normal bovine | GVRI | - | - | |
Blood of normal bovine | GVRI | - | - | |
cells | ||||
cell −1 | GVRI | - | - | |
cell −2 | GVRI | - | - | |
cell −3 | GVRI | - | - | |
Cell-4 | GVRI | - | - | |
cell −5 | GVRI | - | - | |