Figure 2From: Constitutive phosphorylation of the mTORC2/Akt/4E-BP1 pathway in newly derived canine hemangiosarcoma cell linesCell growth under standard conditions. Growth curves of canine HSA cell lines. Each cell line was plated at 5000 cells/well in 1 mL of Medium 199 containing 10% FBS. The cells were trypsinized and counted with a hemocytometer using trypan blue every 24 h.Back to article page