Enhancement of Th1-biased protective immunity against avian influenza H9N2 virus via oral co-administration of attenuated Salmonella enterica serovar Typhimurium expressing chicken interferon-α and interleukin-18 along with an inactivated vaccine

Table 1 PCR primers for amplification of chIFN-α, chIL-18, AIH9, IFN-γ, IL-4, and GAPDH

Target gene		Primer sequence (5′-3′)	Accession no.	Reference
chIFN-α ^a	F	ATGGCTGTGCCTGCAAGCCCA	DQ026259.1	-
	R	CTAAGTGCGCGTGTTGCCTGT
chIL-18 ^b	F^c	GAATTCGCCTTTTGTAAGGATAAAACT	HM854281.1	-
	R^c	AAGCTTTCAGTGATGGTGATGGTGATG TAGGTTGTGCCTTTC
AIH9	F	CTACTGTTGGGAGGAAGAGAATGGT	AF461510.1	[29]
	R	TGGGCGTCTTGAATAGGGTAA
IFN-γ	F	CAAAGCCGCACATCAAACA	X99774	[30]
	R	TTTCACCTTCTTCACGCCATC
IL-4	F	GAGAGGTTTCCTGCGTCAAG	FJ907790.1	[31]
	R	TGGTGGAAGAAGGTACGTAGG
GAPDH	F	AGAACATCATCCCAGCGTCC	X01578	[30]
	R	CGGCAGGTCAGGTCAACA

^aThe primer pair specific for chIFN-α gene was designed using chIFN-α6 nucleotide sequences (Genbank accession number DQ026259.1), and the sequences of the two primers were checked using NCBI Blast Software.
^bThe primer pair specific for chIL-18 gene was designed using chIL-18 nucleotide sequences (Genbank accession number HM854281.1), and the sequences of the two primers were checked using NCBI Blast Software.
^cThe forward and reverse primers specific for chIL-18 gene contain EcoRI and HindIII restriction sites as indicated by the underline. The reverse primer also contained 6×His-Tag sequences indicated in boldface.

ISSN: 1746-6148