Table 2 Respiratory pathogens tested for and tests used [1, 16, 17, 19–22]
From: BPEX Pig Health Scheme: a useful monitoring system for respiratory disease control in pig farms?
Pathogen | Test type | Test manufacturer | Information |
|---|---|---|---|
APP | ELISA for serotypes 3,6 & 8 | Swinecheck® (Biovet, Saint-Hyacinthe, Quebec, Canada) | Sensitivity: 90 to 99% Specificity: 95 to 99% |
PCV2 | PCR | Protocol by Yang et al. 2007 | Detection threshold: 103-1011 million copies DNA per reaction |
PRRSV (unvaccinated farms) | ELISA | BioBest Elisa (Biobest laboratories, United Kingdom) | Sensitivity: > 95% and Specificity: > 95% |
PRRSV (vaccinated farms) | RT-PCR | AcuPig® PRRSV (AnDiaTec GmbH & Co., Germany) | Detection threshold: 100 to 200 viral particles in 1 ml blood |
SI | Haemagglutination inhibition (HI) test | Veterinary Laboratories Agency, United Kingdom (OIE reference laboratory) | Office International des Epizooties (OIE) standard |