Sparse PrPScaccumulation in the placentas of goats with naturally acquired scrapie

BMC Veterinary Research

Table 2 Immunoassays for PrP^Sc in shed cotyledons from sheep and goats with naturally occurring scrapie

	Immunohistochemistry		ELISA		PTA-WB^a
Placenta	No. PrP^Sc-pos cotyledons /no. tested	Relative abundance of PrP^{Sc b}	No. PrP^Sc-pos cotyledons /no. tested	mean A₄₅₀^c (SD)	No. PrP^Sc-pos cotyledons /no. tested
G569	8/9	+2	2/2	2.73 (0.23)	2/2
G736A	2/10	+1	0/7		6/6
G736Z	0/10	0	0/5		0/6
G742	11/11	+1	0/10		2/3
G797	3/10	+1	5/5	0.66 (.21)	7/7
G836	3/20	+1	0/12		1/6
S345	28/28	+1	4/4	2.82 (.27)	2/2
S630	2/2	+4	2/2	3.42 (.13)	2/2
S738	6/6	+4	3/3	3.44 (.18)	2/2
S811	6/6	+4	6/6	3.32 (.24)	2/2
S758	6/6	+4	3/3	3.08 (.54)	1/1
S796	3/3	+4	3/3	3.62 (.19)	4/4
S850	6/6	+1	6/6	0.43 (.07)	2/2

^aHomogenates of individual cotyledons were precipitated with phosphotungstic acid and analyzed by western blot using a combination of mAb F99/97.6.1 and P4.
^bOne section each from the cotyledons with PrP^Sc immunolabeling was evaluated for the percentage of low power (10X) fields with PrP^Sc labelling and considered to have an abundance of 0 if no fields were positive, +1 if 10% or fewer of the fields were PrP^Sc positive, +2 if 11-25% were positive, +3 if 26-50% were positive, +4 if 51 = 75% were positive, and +5 if >76% were positive.
^cMean (SD) values for homogenates of cotyledons (number shown in the previous column), each tested in triplicate. Values below the cut-off limit for the assay are considered negative and not shown.

ISSN: 1746-6148