Table 2 Virulence genes and PCR primers used to test screen Salmonella isolates.
Virulence gene | Pathogenicity island | Gene function | Broad action | Primer sequence (5' to 3') |
|---|---|---|---|---|
prgH | SPI-1 | Type III secretion system apparatus | Invasion of macrophages | F: GCCCGAGCAGCCTGAGAAGTTAGAAA R: TGAAATGAGCGCCCCTTGAGCCAGTC |
sopB | SPI-1 | Type III secreted effector protein | Invasion of macrophages | F: CGGACCGCCCAGCAACAAAACAAGAAGAAG R: TAGTGATGCCCGTTATGCGTCAGTGTATT |
sopE | SPI-1 | Type III secreted effector protein | Invasion of macrophages | F: TCAGTTGGAATTGCTGTGGA R: TCCAAAAACAGGAAACCACAC |
invA | SPI-1 | Type III secretion system apparatus | Invasion of macrophages | F: CTGGCGGTGGGTTTTGTTGTCTTCTCTATT R: AGTTTCTCCCCCTCTTCATGCGTTACCC |
sitC | SPI-1 | Iron transport | Invasion of macrophages/iron acquisition | F: CAGTATATGCTCAACGCGATGTGGGTCTCC R: CGGGGCGAAAATAAAGGCTGTGATGAAC |
spiC | SPI-2 | Type III secretion system | Survival in macrophages | F: CCTGGATAATGACTATTGAT R: AGTTTATGGTGATTGCGTAT |
sifA | SPI-2 | Type III secreted effector protein | Survival in macrophages | F: TTTGCCGAACGCGCCCCCACACG R: GTTGCCTTTTCTTGCGCTTTCCACCCATCT |
misL | SPI-3 | Involved in intramacrophage survival | Survival in macrophages | F: GTCGGCGAATGCCGCGAATA R: GCGCTGTTAACGCTAATAGT |
orfL | SPI-4 | Adhesin/autotransporter | Survival in macrophages/colonisation | F: GGAGTATCGATAAAGATGTT R: GCGCGTAACGTCAGAATCAA |
pipD | SPI-5 | Type III secreted effector-associated with SPI-1 system | Enteritis | F: CGGCGATTCATGACTTTGAT R: CGTTATCATTCGGATCGTAA |
iroN | NA | Siderophore (iron acquisition) | Associated with iron usage | F: ACTGGCACGGCTCGCTGTCGCTCTAT R: CGCTTTACCGCCGTTCTGCCACTGC |
pefA | NA | Fimbriae | Movement | F: GCGCCGCTCAGCCGAACCAG R: CAGCAGAAGCCCAGGAAACAGTG |