Identification of immune genes and proteins involved in the response of bovine mammary tissue to Staphylococcus aureus infection

Table 5 Oligonucleotide primer sequences used for qRT-PCR and recombinant protein expression

Gene	Accession number	Forward (5'→3')	Reverse (5'→3')	Size (bp)	Ref.
RPLP0	BT021080	CAACCCTGAAGTGCTTGACAT	AGGCAGATGGATCAGCCA	220	[12]
IL-1β	M35589	AAATGAACCGAGAAGTGGTGTT	TTCCATATTCCTCTTGGGGTAGA	185	[12]
IL-6	X57317	CTGGGTTCAATCAGGCGAT	CAGCAGGTCAGTGTTTGTGG	206	[12]
IL-8	AF232704	CTGTGTGAAGCTGCAGTTCT	TAAGCAGACCTCGTTTCCAT	180	[12]
TNFα	NM_173966	CTGGTTCAGACACTCAGGTCCT	GAGGTAAAGCCCGTCAGCA	183	[12]
CD14	D84509	GGTGCTACCCGATGTGTCTG	AAGGGATTTCCGTCCAGAGT	191	[12]
CSN1S1	M38641	CCTGTCTTGTGGCTGTTGCTCTTG	CATCTTCCTTTTGAATGTGCTTCTGCT	282	pc*
CSN2	M16645	TCTGCCTCTGCTCCAGTCTT	AGGAGGGGGCATTCACTTT	251	pc*
S100A12	NM_174651	CATTTCGACACCCTCAACAA	CTGTTTTCAGCACCCTGGAC	184
rS100A12	NM_174651	CCGGATCCACTAAGCTGGAAGATCACCTGGAGG	CCAAGCTTTACTCTTTGTGGATATCTATGTGGGCTG	-
PTX3	NM_001076259	TATGCCATGGTGCTTTCAGA	CCAATGAACAATGGACAACAA	182
rPTX3	NM_001076259	ATGGATCCGAGAACTCAGATGATTATGAGCTCATGTA	AGAAGCTTTTAATAAACATACTGTGCTCCTCGTG	-
rtnPTX3	NM_001076259	GTGGATCCGGAGAACTCAGATGATTATGAGCTCATGTA	AGAAGCTTTTAATAAACATACTGTGCTCCTCGTG	-

ISSN: 1746-6148